GFP Antikörper (Green Fluorescent Protein) (AA 1-238)

Details for Product anti-GFP Antibody No. ABIN371877, Anbieter: Anmelden zum Anzeigen
Antigen
  • green fluorescent protein
  • gfp
Epitop
AA 1-238, N-Term
41
31
27
18
5
4
3
3
2
1
1
1
1
1
1
Reaktivität
Aequorea victoria
638
16
5
5
2
2
Wirt
Maus
339
171
80
40
16
9
9
2
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser GFP Antikörper ist unkonjugiert
33
31
28
14
12
12
12
9
9
8
8
7
7
6
6
6
6
6
5
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Applikation
Enzyme Immunoassay (EIA), Western Blotting (WB)
536
354
202
156
145
134
96
87
73
24
15
13
13
8
8
6
6
5
4
4
3
3
3
3
3
2
2
2
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Recombinant GFP (1-238 aa) purified from E. coli
Klon AT2G5
Isotyp IgG2a
Produktmerkmale Synonyms: Green fluorescent protein, GFP-Tag
Reinigung Protein-G affinity chromatography
Andere Bezeichnung GFP
Substanzklasse Tag
Hintergrund GFP, also known as green fluorescent protein, is a protein produced by the jellyfish (Aequorea Victoria) that emits bioluminescence in the green zone of the visible spectrum. GFP has become a useful and ubiquitous tool for making chimeric proteins, where it functions as a fluorescent protein tag. It has been expressed in most known cell types and is used as a noninvasive fluorescent marker in living cells and organisms. This protein enables a wide range of applications where it has functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.Synonyms: GFP-Tag, Green fluorescent protein
UniProt P42212
Forschungsgebiet Tags/Labels
Applikationshinweise ELISA. Western blot (1: 1,000 - 1: 2,000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1.0 mg/mL
Buffer PBS, pH 7.4, containing 0.09 % sodium azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody undiluted at 2-8 °C for up to two weeks or (in aliquots) at -20 °C forlonger. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Haltbarkeit 12 months
Bilder des Herstellers
Western Blotting (WB) image for anti-Green Fluorescent Protein (GFP) (AA 1-238), (N-Term) antibody (ABIN371877) anti-Green Fluorescent Protein (GFP) (AA 1-238), (N-Term) antibody
Western Blotting (WB) image for anti-Green Fluorescent Protein (GFP) (AA 1-238), (N-Term) antibody (ABIN371877) anti-Green Fluorescent Protein (GFP) (AA 1-238), (N-Term) antibody (Image 2)
Allgemeine Veröffentlichungen Phillips: "Green fluorescent protein--a bright idea for the study of bacterial protein localization." in: FEMS microbiology letters, Vol. 204, Issue 1, pp. 9-18, 2001 (PubMed).

Tsien: "The green fluorescent protein." in: Annual review of biochemistry, Vol. 67, pp. 509-44, 1998 (PubMed).

Prasher, Eckenrode, Ward, Prendergast, Cormier: "Primary structure of the Aequorea victoria green-fluorescent protein." in: Gene, Vol. 111, Issue 2, pp. 229-33, 1992 (PubMed).

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