Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) Antikörper

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Antigen
  • erk1/2
  • mitogen-activated protein kinase
  • erk1/2
Reaktivität
Human
403
313
301
57
24
15
9
8
8
8
7
6
5
5
3
3
2
2
2
1
1
1
1
Wirt
Maus
332
75
6
Klonalität (Klon)
Monoklonal ()
Konjugat
Unkonjugiert
12
11
11
9
7
7
7
6
6
6
6
6
6
6
2
2
2
1
1
1
1
1
1
Applikation
Immunoprecipitation (IP), Western Blotting (WB)
291
106
81
78
61
53
39
38
14
10
6
4
3
2
2
2
2
Optionen
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Immunogen Human recombinant Erk1 and Erk2.
Klon SB46b
Isotyp IgG2a
Spezifität This antibody precipitates ~42 kDa and ~44 kDa bands, corresponding to Erk1 and Erk2, respectively.
Produktmerkmale Synonyms: MAPK1, ERK2, PRKM1, PRKM2, ERK-2, ERT1, ERT2, PRKM3, MAPK3, ERK-1, ERK1,Mitogen-activated protein kinase, Extracellular signal-regulated kinase, Insulin-stimulatedMAP2 kinase
Reinigung Purified
Andere Bezeichnung ERK1 / ERK2 (MAPK1/3 Antibody Abstract)
Hintergrund Erk1 and Erk2 are closely related mitogen activated protein (MAP) kinases which are activated by many growth factors, mitogens and differentiation-promoting agents via a protein kinase cascade. Also known as extracellular signal-regulated kinase 1 and 2, p44/p42 MAP kinases, microtubule-associated protein-2 kinases, myelin basic protein (MBP) kinases or EGF receptor T669 (Ert) kinases. (Ref.1-4) Erk1 and Erk2 are ubiquitous and abundant, although their relative abundance in specific tissues may vary. The two kinases are nearly 85 % identical and have higher identity in the core regions involved in substrate binding. (Ref.4) Erk1 and Erk2 are activated approximately 1000-fold by phosphorylation within a Thr-Glu-Tyr motif in the activation loop on both threonine and tyrosine residues by Mek1 and Mek2. (Ref.4,5) Both sites must be phosphorylated for maximum activity. (Ref.1-4) These kinases in turn phosphorylate a variety of different substrates. Erk1 and Erk2 are found in the cytoplasm and are translocated to the nucleus upon activation. Erk1 and Erk2 target membrane proteins, cytosolic proteins, such as downstream kinases, and cytoskeletal proteins and nuclear proteins, such as transcription factors. Many of these substrates are important regulatory proteins. Erk1 and Erk2 represent proximal kinases in the classical kinase pathway which links growth and differentiation signals at the cell surface (through tyrosine kinases) with transcription in the nucleus. (Ref.1-3)Synonyms: ERK-1/ERK-2, Extracellular signal-regulated kinase, Insulin-stimulated MAP2 kinase, MAPK1/MAPK2, Mitogen-activated protein kinase, P42/P44-MAPK
Forschungsgebiet Signaling, Inflammation
Applikationshinweise Western Blot: 1-2 μg/mL. Predicted Mol. Weight: 42 and 44 kDa. Immunoprecipitation: 10-15 μg.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 0.05 mg/mL
Buffer 100 mM Borate buffered saline, pH 8.0 without preservatives.
Konservierungsmittel Without preservative
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Haltbarkeit 12 months
Bilder des Herstellers
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) Antikörper (ABIN371670) anti-Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) antibody
Allgemeine Veröffentlichungen Xu Be, Stippec, Robinson, Cobb: "Hydrophobic as well as charged residues in both MEK1 and ERK2 are important for their proper docking." in: The Journal of biological chemistry, Vol. 276, Issue 28, pp. 26509-15, 2001 (PubMed).

Charest, Mordret, Harder, Jirik, Pelech: "Molecular cloning, expression, and characterization of the human mitogen-activated protein kinase p44erk1." in: Molecular and cellular biology, Vol. 13, Issue 8, pp. 4679-90, 1993 (PubMed).

Boulton, Yancopoulos, Gregory, Slaughter, Moomaw, Hsu, Cobb: "An insulin-stimulated protein kinase similar to yeast kinases involved in cell cycle control." in: Science (New York, N.Y.), Vol. 249, Issue 4964, pp. 64-7, 1990 (PubMed).