Ubiquitin Antikörper

Antigen: Ubiquitin

Synonyme: MGC8385, FLJ25987, Ubc, Ubb2, Uba52, Rps27a, AL033289, CG11624, DmelCG11624, DmUb, Ub, ubi, Ubi-p, ubi-p63E, Ubi-P63e, ubi63E, Ubi63E, Ubi63p, Ubip, Ubiq, Ubp, UBC, CG18282, CG32744, CR32744, DmelCG32744, ubiquitin, si:dz180g5.5, si:busm1-180g5.5, UBB, Ubiquitin, CG11700, ESTS:143G11T, ESTS:199A6T, anon-sts41, poly-ub, DmelCR11700, LOC100194618, UBIQ, LOC100282068

Klonalität: Polyklonal

Wirt: Kaninchen

Applikation: Immunzytochemie (ICC), Immunpräzipitation (IP)

Zertifikate: ISO 9001:2008, ISO 13485:2003

Anbieter: Dako

Produktnummer: ABIN370791

Produktbeschreibung

Immunogen: Ubiquitin isolated from cow erythrocytes and conjungated to chicken gammaglobulins with glutaraldehyde.

Beschreibung: Ubiquitin is a highly conserved 76 amino acid polypeptide with a central role in regulated protein degradation. The ubiquitin/proteasome pathway is the main non-lysosomal route for intracellular protein degradation in eukaryotes, and it plays a key role in many cellular processes, including cell-cycle progression, gene transcription, antigen processing and signal transduction (2-4). Proteins can be modified on lysine residues with single ubiquitin moieties, or be attached to branched multi-ubiquitin chains, the latter targets the proteins to the 26S proteasome: a complex, multicatalytic cytosolic and nuclear protease. Ubiquitin appears to be incorporated into inclusion bodies and protein accumulations characteristic of several of the major neurogenerative diseases including Alzheimer’s disease, Parkinson’s disease and Creutzfeldt Jakob disease (1). Recent findings also substantiate a role of the ubiquitin/proteasome pathway in the regulation of apoptosis (4).

Spezifität: The antibody reacts with human ubiquitin. Traces of contaminating antibodies have been removed by solid-phase absorption with human plasma proteins. In Western blotting of homogenates of endometrial tissues from nonpregnant and pregnant women, the antibody labels bands corresponding to free ubiquitin and ubiquitin conjugates, forming a characteristic high molecular mass “smear” (5). In crossed immunoelectrophoresis, using 12.5 uL antibody per cm 2 gel area against 10 uL of human plasma no precipitate appears. Staining: Coomassie Brilliant Blue. In indirect ELISA, the antibody reacts with ubiquitin coupled to keyhole limpet haemocyanin, but not with keyhole limpet haemocyanin. As demonstrated by immunocytochemistry, the antibody cross-reacts with the ubiquitin-equivalent protein in baboon (5) and wistar rat (8).

Synonyme: MGC8385, FLJ25987, Ubc, Ubb2, Uba52, Rps27a, AL033289, CG11624, DmelCG11624, DmUb, Ub, ubi, Ubi-p, ubi-p63E, Ubi-P63e, ubi63E, Ubi63E, Ubi63p, Ubip, Ubiq, Ubp, UBC, CG18282, CG32744, CR32744, DmelCG32744, ubiquitin, si:dz180g5.5, si:busm1-180g5.5, UBB, Ubiquitin, CG11700, ESTS:143G11T, ESTS:199A6T, anon-sts41, poly-ub, DmelCR11700, LOC100194618, UBIQ, LOC100282068

Anwendungen

Applikationshinweise: Polyclonal Rabbit Anti-Ubiquitin, code No. Z 0458 is intended for use in immunocytochemistry. The antibody is also well-suited for gel immunoprecipitation techniques. In immunocytochemistry, antibodies to ubiquitin may be useful for the investigation of neurodegenerative diseases including Alzheimer's disease, Parkinson's disease and Creutzfeldt Jakob disease (1). Differential identification is aided by the results from a panel of antibodies. Interpretation of results must be made within the context of the patient's clinical history and other diagnostic tests by a certified professional. Introduction: The antibody can be used for labelling paraffin-embedded tissue sections fixed in formalin. Heat- induced epitope retrieval or proteolytic pre-treatment is not required. The tissue should not dry out during the immunocytochemical procedure. Dilution: Polyclonal Rabbit Anti-Ubiquitin, code No. Z 0458, may be used at a dilution range of 1:150-1:300 when applied on formalin-fixed, paraffin-embedded sections of human brain with Alzheimer's disease, and 30 minutes incubation at room temperature with the primary antibody. Optimal conditions may vary depending on specimen and preparation method, and should be determined by each individual laboratory. The recommended negative control is DakoCytomation Rabbit Immunoglobulin Fraction (Solid-Phase Absorbed), code No. X 0936, diluted to the same protein concentration as the primary antibody. Unless the stability in the actual test system has been established, it is recommended to dilute the product immediately before use or dilute in DakoCytomation Antibody Diluent, code No. S 0809. Visualization: DAKO LSAB/HRP kit, code No. K 0679, and DAKO EnVision/HRP kits, code Nos. K 4008 and K 4010, are recommended. Follow the procedure enclosed with the selected visualization kit. Normal tissues: The antibody labels cells in the nonpregnant endometrium in human, particularly the glandular epithelial cells (5). Abnormal tissues: The antibody labelled Lewy bodies in human brain with Parkinson's disease (6). The antibody has been used in the studies of Alzheimer's disease and other neurodegenerative diseases (7). When tested on formalin-fixed, paraffin-embedded sections of human brain with Alzheimer's disease, the antibody labelled the periphery of senile plaques and neurofibrillary tangles.

Buffer: Purified immunoglobulin fraction of rabbit antiserum provided in liquid form. In 0.1 mol/L NaCl, 15 mmol/L NaN 3 .

Lagerung: Store at 2-8 °C. Precautions: 1. For professional users. 2. This product contains sodium azide (NaN 3 ), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used. 4. The product may be used in different techniques and in combination with different sample types and materials, therefore each individual laboratory should validate the test system applied.

Forschungsgebiet: Proteasen, Aminosäuren

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.