S-100 Antikörper

Antigen: S-100

Klonalität: Polyklonal

Wirt: Kaninchen

Applikation: Immunzytochemie (ICC)

Zertifikate: ISO 9001:2008, ISO 13485:2003

Anbieter: Dako

Produktnummer: ABIN370787

Produktbeschreibung

Weitere Bezeichnung: S100

Immunogen: S100 isolated from cow brain.

Beschreibung: S100 is a multigene family of low molecular weight (Mr between 9 000 and 13 000) Ca 2+ -binding proteins. The family comprises 19 members that are differentially expressed in a large number of cell types. Thus, S100B (previously S100 beta) is most abundant in glial cells of the central and peripheral nervous system, in melano-cytes, chondrocytes, and adipocytes, whereas S100A1 (previously S100A/S100 alpha) is most abundant in cardio-myocytes, slow twitch skeletal muscle cells, salivary epithelial cells, and renal cells. Additionally, S100B is found in tumour cells and subpopulations of neurons, while S100A1 has also been detected in hippocampal neurons. S100A6 is expressed by fibroblasts and smooth and heart muscle cells (5). Members of the S100 family have been implicated in the Ca 2+ -dependent regulation of a variety of intracellular activities, e.g. protein phosphorylation, cell proliferation (including neoplastic transformation), and differentiation (7).

Spezifität: The antibody has been solid-phase absorbed with human plasma and cow serum proteins. In crossed immunoelectrophoresis using 50 µL antibody per cm 2 gel area, no reaction with 2 µL human plasma and 2 µL cow serum is observed. The antibody shows one distinct double peak (S100) with human and cow brain extracts. Staining: Coomassie Brilliant Blue. In indirect ELISA, the antibody shows no reaction with human plasma and cow serum. In Western blotting of purified human recombinant S100 proteins, the antibody labels S100B strongly, S100A1 weakly, and S100A6 very weakly. No reaction was observed with the other S100 proteins tested, S100A2, S100A3 and S100A4 (8). As demonstrated by immunocytochemistry on formalin-fixed tissues, the antibody cross-reacts with the S100 equivalent protein in cat, horse, mouse, rat, and swine. Additionally it reacts strongly with human and cow S100.

Anwendungen

Applikationshinweise: Polyclonal Rabbit Anti-S100 is intended for use in immunocytochemistry. The antibody labels cells expressing S100 and is a useful tool for the identification of S100-positive neoplasms, such as malignant melanoma (1, 2), Langerhans' histiocytosis (3), chondroblastoma (4), and schwannoma (5). For differential identification the use of a panel of antibodies is mandatory. A panel of antibodies including Polyclonal Anti-S100, code No. Z 0311, has also been applied by the International Lymphoma Study Group for the classification of tumours of suspected histiocytic/dendritic cell type (6). Interpretation of results must be made within the context of the patient's clinical history and other diagnostic tests by a certified professional. Introduction: The antibody can be used for labelling paraffin-embedded tissue sections fixed in formalin. Heat-induced epitope retrieval in DakoCytomation Target Retrieval Solution, code No. S 1700, or 10 mmol/L citrate buffer, pH 6.0, is recommended. The tissue sections should not dry out during the treatment or the following immunocytochemical staining procedure. Frozen sections and cell preparations: In frozen sections the highly soluble S100 molecule tends to show aberrant distribution or elute from the tissue. Dilution: Polyclonal Rabbit Anti-S100, code No. Z 0311, may be used at a dilution of 1:400 when applied on formalin-fixed, paraffin-embedded sections and using 20 minutes heat-induced epitope retrieval in DakoCytomation Target Retrieval Solution, code No. S 1700, and 30 minutes incubation at room temperature with the primary antibody. Optimal conditions may vary depending on specimen and preparation method, and should be determined by each individual laboratory. The recommended negative control is DakoCytomation Rabbit Immunoglobulin Fraction (Solid-Phase Absorbed), code No. X 0936, diluted to the same protein concentration as the primary antibody. Unless the stability in the actual test system has been established, it is recommended to dilute the product immediately before use or dilute in DakoCytomation Antibody Diluent, code No. S 0809. Visualization: DAKO LSAB/HRP kit, code No. K 0679, and DAKO EnVision /HRP kits, code Nos. K 4008 and K 4010, are recommended. Automation: The antibody is well suited for immunocytochemial staining using automated platforms, such as the DakoCytomation Autostainer. Performance characteristics: Cells labelled by the antibody display staining confined to the cytoplasm. Normal tissues: Positive labelling with the antibody is observed in some Langerhans' cells and melanocytes of the skin, interdigitating reticulum cells in lymph nodes, medullary epithelial reticular cells in the thymus, chondrocytes in cartilagenous tissue, adipocytes in some, but not other biopsies, myoepithelial cells in salivary glands and breast, folliculostellate cells of the pituitary gland, and Schwann cells and glial cells of nervous tissue. Weak labelling is found in epithelial cells of the mammary and sweat glands.

Buffer: Purified immunoglobulin fraction of rabbit antiserum provided in liquid form. In 0.1 mol/L NaCl, 15 mmol/L NaN 3 , pH 7.2.

Lagerung: Store at 2-8 °C. Precautions: 1. For professional users. 2. This product contains sodium azide (NaN 3 ), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.

Forschungsgebiet: Zellzyklus

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.