zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antikörper

Antigen: zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70)

Synonyme: SRK, STD, TZK, ZAP-70, FLJ17670, FLJ17679, Srk, mur, 70kDa, mrtle, AI327364, MGC108902, zap70, MGC80670, MGC88962, ZAP70

Klonalität: Monoklonal (2F32)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p))

Zertifikate: ISO 9001:2008, ISO 13485:2003

Anbieter: Dako

Produktnummer: ABIN370768

Produktbeschreibung

Weitere Bezeichnung: ZAP-70

Immunogen: Recombinant ZAP-70 protein including residues 1-254 and encompassing SH2 domains of human ZAP-70 (7).

Isotyp: IgG2a, kappa chain  (Passende Sekundärantikörper)

Klon: 2F32

Beschreibung: ZAP-70 (zeta–associated protein of 70 kDa) is a tyrosine kinase of the Syk family that plays a critical role in triggering the signaling cascade in response to T-cell receptor stimulation (1). It is primarily expressed in T cells and natural killer (NK) cells (1-6), but is also detectable in mast cells and basophils (2). ZAP-70 is also expressed in normal pro/pre B cells but not in normal mature B cells (4). ZAP-70 is expressed in B cells in a subset of chronic lymphocytic leukemias (CLL) closely associated with an unmutated configuration of the immunoglobulin heavy-chain variable region (IgV H ) genes (1-3, 5, 6). The precise functional significance of ZAP-70 in this subset of CLL B cells is unknown. Some data indicates that expression of ZAP-70 in CLL allows more effective IgM signaling in CLL B cells, which could explain the disease progress (5). Also, some other B-cell neoplasms (1, 6) and T-cell neoplasms express ZAP-70 (6).

Spezifität: In Western blotting of whole cell lysates of normal peripheral blood mononuclear cells, the antibody labels a band corresponding to ZAP-70 (2). In Western blotting of whole cell lysates of CD19-positive purified leukemia cells from patients with Ig-unmutated and Ig-mutated CLL, the antibody labels a band corresponding to ZAP-70 in the Ig-unmutated CLL samples, whereas no band is observed in the Ig-mutated CLL samples (2). In Western blotting of cell lysates of Jurkat cells (T-lymphoblastic cell line), the antibody labels a band of 70 kDa protein (6). In Western blotting of cell lysates of A431 cells (carcinoma cell line), no band is observed (6).

Anwendungen

Applikationshinweise: Paraffin sections: The antibody can be used for labeling paraffin-embedded tissue sections fixed in formalin, including tissues decalcified in 85% formic acid. Pre-treatment of deparaffinized tissue sections with heat-induced epitope retrieval (HIER) is required. Optimal results are obtained by pretreating tissues with HIER using Dako Target Retrieval Solution. Dilution: Monoclonal Mouse Anti-Human ZAP-70 ( Cells labeled by the antibody display both cytoplasmic and nuclear staining. Normal tissues: The antibody labels T cells in lymphoid tissues such as tonsil and gut-associated lymphoid tissue, as well as in other tested tissues, including colon, kidney, liver, pancreas and prostate. In addition to T cells, the antibody prominently labels the epithelial cell cytoplasma of distale tubuli and collecting ducts in kidney.

Buffer: Monoclonal mouse antibody provided in liquid form as cell culture supernatant (containing fetal bovine serum) dialyzed against 0.05 mol/L Tris/HCl, pH 7.2, and containing 0.015 mol/L sodium azide.

Lagerung: Store at 2-8 °C. Precautions: 1. For professional users. 2. This product contains sodium azide (NaN 3 ), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used. 4. Wear appropriate Personal Protective Equipment to avoid contact with eyes and skin. 5. Unused solution should be disposed of according to local, State and Federal regulations.

Forschungsgebiet: Signaltransduktion, Tags/Labels, Immunologie, Krebs

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.