Antigen Identified By Monoclonal Antikörper Ki-67 (MKI67) Antikörper

Antigen: Antigen Identified By Monoclonal Antibody Ki-67 (MKI67)

Synonyme: KIA, Ki67, Ki-67, D630048A14Rik, MKI67, kia, mki67, MGC132156

Klonalität: Monoklonal (TEC-3)

Wirt: Ratte

Reaktivität: Maus

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p))

Zertifikate: ISO 9001:2008, ISO 13485:2003

Anbieter: Dako

Produktnummer: ABIN370746

Produktbeschreibung

Weitere Bezeichnung: Ki-67 Antigen

Immunogen: Parts of mouse Ki-67-equivalent cDNA bacterially expressed as recombinant protein.

Isotyp: IgG2a

Klon: TEC-3

Beschreibung: The Ki-67 antigen is a large nuclear protein preferentially expressed during all active phases of the cell cycle (G 1 , S, G 2 and M phases), but absent in resting cells (G 0 ) cells. Antibodies against the Ki-67 antigen have proven valuable by allowing direct monitoring of the growth fraction of a pool of cells (1). The TEC-3 antibody is the antibody of choice for the demonstration of the Ki-67 antigen in formalin-fixed, paraffin-embedded mouse specimens.

Spezifität: The antibody labels the Ki-67 antigen in mouse cells. The antibody does not cross-react with the human Ki-67 antigen.

Anwendungen

Applikationshinweise: Paraffin sections: The antibody can be used for labelling paraffin-embedded tissue sections fixed in formalin. Pre-treatment of tissues with heat-induced epitope retrieval is required. Optimal results are obtained with DakoCytomation Target Retrieval Solution, Citrate pH 6, code No S2369. Pre-treatment of tissues with Proteinase K was found inefficient. The tissue sections should not dry out during the treatment or during the following immunocytochemical staining procedure. Dilution: Monoclonal Rat Anti-Mouse Ki-67 Antigen, code No. M 7249, may be used at a dilution range of 1:25-1:50 when applied on formalin-fixed, paraffin-embedded sections of mouse tissue using 20 minutes heat-induced-epitope retrieval in DakoCytomation Target Retrieval Solution, Citrate pH 6, code No S2369, and 30 minutes incubation at room temperature with the primary antibody. Optimal conditions may vary depending on specimen and preparation method, and should be determined by each individual laboratory. Visualization: For immunocytochemistry on mouse tissue, the combination of Biotinylated Rabbit Anti-Rat Immunoglobulins (DakoCytomation, code No. E0468) diluted 1:200, and Streptavidin/HRP (DakoCytomation, code No. P0397) diluted 1:300 is recommended for visualization using DAB+ (DakoCytomation, code No. K3467) as chromogen. Performance characteristics: Cells labelled by the antibody display a nuclear staining pattern. (112401-002) M7249/RUO/KLI/30.03.05 p. 2/2

Buffer: Monoclonal rat antibody provided in liquid form as cell culture supernatant dialysed against 0.05 mol/L Tris/HCl, pH 7.2, and containing 15 mmol/L NaN 3 .

Lagerung: Store at 2-8 °C. Precautions: 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains sodium azide (NaN 3 ), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.

Forschungsgebiet: Proliferationsmarker, Zellzyklus

Beschränkungen: Nur für Forschungszwecke einsetzbar