Cyclin D1 (CCND1) Antikörper

Antigen: Cyclin D1 (CCND1)

Synonyme: BCL1, PRAD1, U21B31, D11S287E, cD1, Cyl-1, bcl-1, AI327039, DIP1, GCIP, Maid, SECC-8, SSEC-8, AU022347, cb161, cycd1, fb52e01, fc83a12, id:ibd1198, wu:fb52e01, wu:fc83a12, MGC109078, ccnd1b, MGC52909, MGC132138, ccnd1, MGC53090, bcl1, prad1, u21b31, MGC69515, d11s287e, CCND1, ccndbp1, MGC137744, ZHM, CCNDBP1, MGC133757, DKFZp468E2216, DKFZp469C1216, DKFZp469H0317

Klonalität: Monoklonal (SP4)

Wirt: Kaninchen

Reaktivität: Human

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p))

Zertifikate: ISO 9001:2008, ISO 13485:2003

Anbieter: Dako

Produktnummer: ABIN370685

Produktbeschreibung

Weitere Bezeichnung: Cyclin D1

Immunogen: A synthetic peptide from C-terminus of human Cyclin D1

Klon: SP4

Beschreibung: Synonyms for antigen PRAD-1, CCND1, Bcl-1 (4) Summary and explanation Cyclin D1 is a 36 kD protein encoded by the CCND1 (bcl-1) gene located on chromosome 11q13. Cyclin D1 associates with and activates cyclin-dependent kinases (CDKs) CDK4 and CDK6 (4-6). The protein functions as a CDK-dependent regulator of the cell cycle by phosphorylating and inactivating the retinoblastoma protein, thereby allowing for progression through the G1-S phase of the cell cycle (4,6). Cyclin D1 is also involved in cell cycle or CDK-independent functions including associating with and regulation of a variety of transcription factors and transcriptional coregulators. Cyclin D1 has also been shown to be involved in the regulation of cellular metabolism, fat cell differentiation and cell migration (4,6). Cyclin D1 overexpression is associated with tumorigenesis, and Cyclin D1 amplification and/or overexpression have been demonstrated in a variety of human tumors, including mantle cell lymphomas, breast carcinomas, head and neck squamous cell carcinomas and esophageal cancers (4).

Spezifität: In Western blotting of EL4 cell lysates the antibody labels a major band of approximately 36 kD, corresponding to the expected molecular weight of Cyclin D1 (5,7).

Synonyme: BCL1, PRAD1, U21B31, D11S287E, cD1, Cyl-1, bcl-1, AI327039, DIP1, GCIP, Maid, SECC-8, SSEC-8, AU022347, cb161, cycd1, fb52e01, fc83a12, id:ibd1198, wu:fb52e01, wu:fc83a12, MGC109078, ccnd1b, MGC52909, MGC132138, ccnd1, MGC53090, bcl1, prad1, u21b31, MGC69515, d11s287e, CCND1, ccndbp1, MGC137744, ZHM, CCNDBP1, MGC133757, DKFZp468E2216, DKFZp469C1216, DKFZp469H0317

Anwendungen

Applikationshinweise: Paraffin sections: The antibody can be used for labeling paraffin-embedded tissue sections fixed in formalin or B5 (2). Pre-treatment of deparaffinized tissue sections with heat-induced epitope retrieval (HIER) is required. Optimal results are obtained by pretreating tissues with HIER using Dako Target Retrieval Solution, pH 9 ( Dilution: Monoclonal Rabbit Anti-Human Cyclin D1, The cellular staining pattern is predominantly nuclear. Normal tissues (8): Tissue Type (# tested) Positively Staining Tissue Elements Bone Marrow (2) 0/2 Breast (2) 2/2 Ductal epithelial cells, (1%) nuclear and (25%) nuclear and cytoplasmic Cervix (2) 1/2 Basal epithelial cells (30%), nuclear Mesothelial Cells (3) 0/3 Nerve (2) 0/2 Ovary (3) 0/3 Parathyroid (3) 1/3 Glandular epithelial cells (<1%), nuclear Prostate (2) 1/2 Glandular epithelial cells (10%), nuclear Salivary gland (3) 1/3 Glandular epithelial cells, (50%) nuclear and (5%) nuclear and cytoplasmic Small intestine (3) 1/3 Glandular epithelial cells and macrophages (1%), nuclear Thyroid (2) 0/2 Tonsil (3) 2/3 Basal epithelial cells (80%), nuclear 1/2 Stromal cells (1%), nuclear Uterus (2) 1/2 Surface epithelial cells (5%), nuclear Abnormal tissues (1,3): Tissue Type (# tested) Positively Staining Tumors Mantle cell lymphoma (42) 1,3 42/42 Hairy cell leukemia (5) 3 2/5 Chronic lymphocytic leukemia/ small lymphocytic lymphoma (27) 1,3 2/27 Diffuse large B-cell lymphoma (25) 3 0/25 Extranodal marginal zone lymphoma of MALT(14) 3 0/14 Follicular lymphoma (16) 1,3 0/16 Marginal zone lymphoma (9) 1,3 0/9 Lymphoplasmacytic lymphoma (1) 3 0/1 Lymphoblastic lymphoma (1 T and 1 B lineage) (2) 3 0/2 Extranodal NK/T-cell lymphoma (13) 3 0/13 Peripheral T-cell lymphoma, NOS (6) 3 0/6 Anaplastic large cell lymphoma (1) 3 0/1 Angioimmunoblastic T-cell lymphoma (1) 3 0/1 Multiple Myeloma (14) 1,3 5/14 Hodgkin lymphoma (4) 3 0/4 (115837-001) 307003EFG_001 p. 3/7

Buffer: Monoclonal Rabbit antibody provided in liquid form as cell culture supernatant and containing 0.015 mol/L sodium azide. This product contains stabilizing protein.

Lagerung: Store at 2-8 °C. Precautions: 1. For professional users. 2. This product contains sodium azide (NaN 3 ), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used. 4. Wear appropriate Personal Protective Equipment to avoid contact with eyes and skin. 5. Unused solution should be disposed of according to local, State and Federal regulations.

Forschungsgebiet: Zellzyklus

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Bilder

Mantle cell lymphoma. The neoplastic cells show a moderateto strong nuclear staining reaction.

B-cell chronic lymphocytic leukemia/small lymphocytic lymphoma.The neoplastic cells are negative, and only scattered endothelialcells show a nuclear staining reaction.

Tonsil. The suprabasal layer of the squamous epithelium showsa moderate to strong nuclear staining reaction.