L523S Protein Antikörper

Antigen: L523S Protein

Klonalität: Monoklonal (691)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p))

Zertifikate: ISO 9001:2008, ISO 13485:2003

Anbieter: Dako

Produktnummer: ABIN370671

Produktbeschreibung

Immunogen: Purified recombinant protein, encoding amino acids 2-580 (4).

Isotyp: IgG2a, kappa chain  (Passende Sekundärantikörper)

Klon: 691

Beschreibung: Synonyms for antigen KOC (K homology domain containing protein overexpressed in cancer), L523S, IGF2BP3 Summary and explanation IMP3 (insulin-like growth factor II mRNA-binding protein 3) is a 580 amino acid oncofetal RNA binding protein containing four K homology domains which is encoded by a 4350 bp mRNA transcript produced by the IGF2BP3 gene on chromosome 7p11.5 (1,2). This 65-70 kDa protein regulates insulin-like growth factor II (IGF-II) transcripts during embryogenesis and is later re-expressed in a proportion of neoplastic cells from various tumor types including lung squamous cell carcinoma and lung and pancreatic adenocarcinoma (3-6). IMP3 was originally identified during an analysis of differentially expressed genes comparing pancreatic cancer to normal pancreas (1). Subsequently, IMP3 was isolated using cDNA library subtraction and microarray technology as preferentially expressed in lung cancer compared to normal lung (7). IMP3 transcripts have also been detected in fine needle aspirates of patients diagnosed with colorectal and pancreatic carcinoma (8). mRNA expression was found to be minimal in many normal tissues using rtPCR and microarray analysis (5). A subset of tumors have been found to express IMP3 protein including melanoma, mesothelioma and carcinomas from the bladder, cervix, colorectum, esophagus, liver, lung, pancreas, stomach, and uterus (9-19).

Spezifität: Internal studies demonstrated a 70 kDa band in Western blots of human lung carcinoma cell lysate A549 using anti-IMP3, corresponding to the expected molecular weight of the IMP3 protein (1, 5). A faint 48 kDa lower molecular weight band was also identified. Monoclonal mouse anti-IMP3 was also tested using an ELISA assay to detect immunoreactivity with the recombinant IMP3 protein.

Anwendungen

Applikationshinweise: Paraffin sections: The antibody can be used for labeling paraffin-embedded tissue sections fixed in formalin. Pretreatment of tissue with proteolytic enzymes is not recommended. The deparaffinized tissue sections must be treated with heat prior to the IHC staining procedure. Heat-induced epitope retrieval (HIER) involves immersion of tissue sections in a pre-heated buffer solution and maintaining heat in a water bath (95-99 °C). Alternative heat sources m ay be used for HIER upon validation against the recommended procedure. Use a 40-minute heating protocol for HIER performed at 95-99 °C, after thermal treatment, a llow the jar with buffer and slides to cool for 20 minutes at room temperature. Rinse well with buffer or deionized water following HIER. Target Retrieval Solution pH 9 ( Dilution: Monoclonal Mouse Anti-Human IMP3, The cellular staining pattern for anti-IMP3 is predominantly cytoplasmic, with rare nuclear and membrane staining (4, 20). Normal tissues (20): Anti-IMP3 immunostains a variety of normal cell types, however the staining intensity of the majority of positive cell types is generally low. Intense immunostaining has been observed in oocytes of the ovary, glandular epithelium of the stomach and squamous epithelium and germinal center cells in the tonsil (20). Tissue Type (# tested) Positively Staining Tissue Elements Adrenal (3) 3/3 Adrenal cortical cells (<5-50%), cytoplasmic Breast (3) 3/3 Ductal and lobular epithelial cells (<5-20%), cytoplasmic Bone Marrow (3) 0/3 Brain/Cerebellum (3) 3/3 Granular layer cells (100%), cytoplasmic Brain/Cerebrum (3) 0/3 Cervix (3) 1/3 Endocervical cells (100%), cytoplasmic Colon (3) 3/3 Glandular epithelial cells (70%), cytoplasmic Esophagus (3) 1/3 Squamous mucosal cells (<5%), cytoplasmic Heart (3) 0/3 Kidney (3) 0/3 Liver (3) 0/3 Lung (3) 3/3 Bronchial epithelial cells (90%), cytoplasmic Nerve (3) 0/3 Ovary (3) 2/3 Oocytes (100%), cytoplasmic and nuclear,1/3 granulosa cells (100%), cytoplasmic Pancreas (3) 3/3 Islet cells 2/3 (100%), cytoplasmic and membrane, 1/3 acini (30%), cytoplasmic Parathyroid (3) 0/3 Pericardial mesothelial cells (2) 2/2 (100%), cytoplasmic Pituitary (3) 3/3 Pituicytes (20-60%), cytoplasmic Prostate (3) 0/3 Salivary gland (3) 0/3 Skeletal muscle (3) 0/3 (114335-003) 306573EFG_004 p. 3/10 Skin (3) 1/3 Hair follicle epithelial cells (40%), cytoplasmic Small intestine (3) 0/3 Spleen (3) 0/3 Stomach (3) 3/3 Glandular epithelial cells (<10-30%), cytoplasmic Testis (3) 3/3 Spermatogonia (50%), Sertoli cells (90%), cytoplasmic and nuclear Thymus (3) 3/3 Thymic epithelial cells (100%), cytoplasmic Thyroid (3) 0/3 Tonsil (3) 3/3 Germinal center cells (70%), squamous epithelial cells (30%), cytoplasmic Uterus (3) 2/3 Endometrial cells (<5-60%), cytoplasmic Abnormal tissues (4,9-19): Tissue Type (# tested) Positively Staining Tumors Anal carcinoma (10) 2/10 Bladder carcinoma (115) 58/115 Breast carcinoma (26) 2/26 Cervical carcinoma (9) 5/9 Cholangiocarcinoma (4) 3/4 Colorectal carcinoma (37) 18/37 Esophageal carcinoma (14) 10/14 Endometrial carcinoma (174) 77/174 Liver carcinoma (395) 259/395 Lung carcinoma (30) 22/30 Melanoma (27) 23/27 Mesothelioma (11) 10/11 Neuroendocrine carcinoma, extrapulmonary (53) 47/53 Neuroendocrine carcinoma, pulmonary (14) 14/14 Pancreatic carcinoma (98) 91/98 Parathyroid carcinoma (3) 0/3 Renal cell carcinoma (24) 3/24 Salivary gland carcinoma (10) 0/10 Stomach carcinoma (25) 15/25 Thyroid gland carcinoma (24) 4/24 Uterine cervical carcinoma (44) 41/44

Buffer: Monoclonal mouse antibody provided in liquid form as cell culture supernatant (containing fetal bovine serum) dialyzed against 0.02 mol/L PBS, pH 6.0, and 0.015 mol/L sodium azide. This product contains stabilizing protein.

Lagerung: Store at 2-8 °C. Precautions: 1. For professional users. 2. This product contains sodium azide (NaN 3 ), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used. 4. Wear appropriate Personal Protective Equipment to avoid contact with eyes and skin. 5. Unused solution should be disposed of according to local, State and Federal regulations.

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.