D240 Antikörper

Antigen: D240

Klonalität: Monoklonal (D2-40 1)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (IHC), Immunhistochemie (IHC)

Zertifikate: ISO 9001:2008, ISO 13485:2003

Anbieter: Dako

Produktnummer: ABIN370664

Produktbeschreibung

Weitere Bezeichnung: D2-40

Immunogen: Dysgerminoma tissue 1 Immunogène : Tissu dysgerminome 1 Isotype / Isotyp : IgG 1 , kappa

Klon: D2-40 1

Beschreibung: Monoclonal mouse anti-D2-40 identifies a 40 kD O-linked sialoglycoprotein present on a variety of tissues including fetal testes and testicular germ cell tumors. 1 D2-40 has also been demonstrated to react with lymphatic endothelium and is unreactive with vascular endothelium. 2, 3 In neoplastic tissue, immunostaining of lymphatic endothelium by D2-40 has been shown to be useful in identifying lymphatic invasion of primary tumors. 2, 3 Specificity Introduction

Spezifität: Monoclonal anti-D2-40 was tested by Western blotting of cell lysates from a variety of tumor cell lines. D2-40 identified a 40 kD band in immunoblots of HEY ovarian epithelial carcinoma cells and a slightly higher molecular weight form in blots of MGH-U3 cells. D2-40 immunoreactivity has also been observed in Western blots of SK-OV-3 and Caov-3 ovarian epithelial carcinoma cells, PA-1PA-1 ovarian teratocarcinoma cells, Tera-1 and Tera-2 testicular teratocarcinoma cells, 833 embryonal carcinoma cells, RT-4 bladder transitional carcinoma cells and U-2 OS osteosarcoma cells. No reactivity was observed in Western blots of other tumor cell lines derived from a variety of cancers. 1 The effect of enzyme digestion on the immunoreactivity of D2-40 was tested using affinity purified antigen from HEY cells and intact HEY cells. The immunoreactivity of D2-40 with Western blots of purified antigen and with cells by flow cytometry was unaffected by the removal of sialic acid residues through neuraminidase treatment of the cells or protein. Treatment of protein or cells with O-sialoglycoprotease destroyed the immunoreactivity of D2-40 in both Western blotting and flow cytometry, demonstrating that the antigen recognized by D2-40 is an O-linked glycoprotein. 1 provided

Anwendungen

Applikationshinweise: This antibody is intended for laboratory use to identify qualitatively by light microscopy D2-40 expressing cells in normal and neoplastic tissues using immunohistochemical (IHC) test methods. Positive results aid in the identification of lymphatic invasion of a variety of cancers. 2, 3 Interpretation must be made within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist. Refer to the General Instructions for Immunohistochemical Staining or the Detection System Instructions of IHC procedures for: (1) Principle of Procedure, (2) Materials Required, Not Supplied, (3) Storage Paraffin Sections Anti-D2-40 can be used on formalin-fixed, paraffin-embedded tissue sections. Pretreatment of tissue with proteolytic enzymes is not recommended. The deparaffinized tissue sections must be treated with heat prior to the IHC staining procedure. Heat-induced epitope retrieval (HIER) involves immersion of tissue sections in a pre-heated buffer solution and maintaining heat in a water bath or steamer (95-99°C). Use a 20-minute heating protocol for HIER performed at 95-99°C, after thermal treatment, allow the jar with buffer and slides to cool for 20 minutes at room temperature. Rinse well with buffer or deionized water following HIER. For greater adherence of tissue sections to glass slides, the use of Silanized Slides (code S3003) is recommended. Target Retrieval Solution (code S1700) or 10x Concentrate (code S1699) is recommended. Cryostat Sections And Cell Smears Anti-D2-40 can be used for labelling acetone-fixed cryostat sections or fixed cell smears. Follow the recommended procedure for the detection system selected. Staining interpretation The cellular staining pattern for anti-D2-40 is cytoplasmic and sometimes membranous. Normal Tissues 6 Anti-D2-40 strongly immunostains lymphatic endothelium, ganglion cells and nerve in many normal tissu e types. Vascular endothelial cells were negative in all tissues. 6 Tissue Type (# tested) Positively Staining Tissue Elements Adrenal (3) 2/3 cytoplasm of capsular fibroblasts, 1/3 cytoplasm of interstitial fibroblasts, 1/3 cytoplasm of pericapsular lymphocytes Bone marrow (3) 3/3 negative Brain, cerebellum (3) 3/3 cytoplasm of neuropil Brain, cerebrum (3) 3/3 nuclei of rare astrocytes Breast (3) 3/3 cytoplasm of myoepithelial cells Cervix (3) 3/3 membrane and/or cytoplasm of basilar epithelial cells, 3/3 cytoplasm of stromal fibroblasts Colon (3) 2/3 lymphocytes Esophagus (3) 1/3 cytoplasm of ganglion cells in muscle, 1/3 cytoplasm of basilar epithelium Heart (3) 2/3 cytoplasm of myocytes Kidney (3) 1/3 cytoplasm of pericapsular fibroblasts Liver (3) 3/3 negative Lung (3) 2/3 cytoplasm of bronchial epithelial cells, 3/3 cytoplasmic edge of alveolar cells Mesothelial cells (3) 1/3 cytoplasm of mesothelial cells Ovary (3) 3/3 cytoplasm of ovarian stromal cells, 1/3 cytoplasm of surface epithelium of inclusion cysts Pancreas (3) 2/3 cytoplasm of periductal fibroblasts, 1/3 cytoplasm of ductal myoepithelial cells Parathyroid (3) 3/3 negative Peripheral nerve (3) 2/3 cytoplasm of perineurium Pituitary (3) 1/3 cytoplasm of pericapsular fibroblasts in neurohypophysis, 1/3 cytoplasm of neuropil in neurohypophysis Prostate(3) 3/3 cytoplasm of basal cells of ducts, 3/3 cytoplasm of stromal fibroblasts Salivary gland (3) 3/3 cytoplasm of ductal and acinar myoepithelial cells (107695-002) 305832EFG_111204 p. 3/10 Normal Tissues 6 Tissue Type (# tested) Positively Staining Tissue Elements Skeletal muscle (3) 3/3 cytoplasm of perimysium Skin (3) 3/3 cytoplasm of myoepithelial cells of adnexal structures Small intestine (3) 3/3 cytoplasm of myenteric plexus Spleen (3) 3/3 negative Stomach (3) 3/3 cytoplasm of periglandular fibroblasts,1/3 cytoplasm of myenteric plexus, 1/3 cytoplasm and membrane of germinal center lymphocytes Testes (3/3) 3/3 cytoplasm of peritubular fibroblasts Thymus (3) 3/3 cytoplasm and membrane of medullary lymphocytes Thyroid (3) 3/3 negative Tonsil (3) 3/3 cytoplasm and membrane of basilar epithelial cells and germinal center lymphocytes Uterus (3) 3/3 cytoplasm of myometrium, 1/3 cytoplasm of endometrial glands Abnormal Tissues 1, 3 Tissue Type (# tested) Positively Staining Tissue Kaposi's Sarcoma (24): Patch, plaque and nodular stages 24/24 Lymphangioma (10) 10/10 endothelium lining the sinusoidal spaces Angiosarcoma (7) 3/7 Leiomyosarcoma (10) 3/10 Dermatofibroma (6) 2/6 Malignant fibrous histiocytoma (2) 1/2 Hemangioma (10) 0/10 Glomus tumor (3) 0/3 Angiolipoma (2) 0/2 Pyogenic granuloma (2) 0/2 Vascular malformation (2) 0/2 Hemangiopericytoma (1) 0/1 Hemangioendothelioma (1) 0/1 Dermatofibrosarcoma protuberans (4) 0/4 Neurofibromas (6) 0/6 Pure Germ cell tumors Seminomas (35) 34/35 Embryonal carcinomas (5) 1/5 Immature and mature teratoma (1) 0/1 Mixed germ cell tumors Seminomas (12) 12/12 Embryonal carcinomas (21) 17/21 Immature and mature teratoma (16) 5/16 Yolk sac tumor (4) 1/4 Choriocarcinoma (2) 0/2 (107695-002) 305832EFG_111204 p. 4/10

Buffer: Anti-D2-40 is provided in liquid form as tissue culture supernatant (containing fetal bovine serum) dialyzed against 0.05 mol/L Tris-HCl, pH 7.2, and 0.015 mol/L sodium azide. Contains stabilizing protein. M3619 may be used at a dilution 1:100-1:200 when

Lagerung: Store at 2-8 °C. Precautions: 1. For professional users. 2. This product contains sodium azide (NaN 3 ), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, build-ups of NaN 3 may react with lead and copper plumbing to form highly explosive metal azides. Upon disposal, flush with large volumes of water to prevent azide build-up in plumbing. 4, 5 3. Avoid microbial contamination of reagents or increase in nonspecific staining may occur. 4. As with any product derived from biological sources, proper handling procedures should be used. 5. Wear appropriate Personal Protective Equipment to avoid contact with eyes and skin. 6. Unused reagents should be disposed of according to local, state, and federal regulations. 7. Safety Data Sheet available for professional users on request.

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.