N-Cadherin (CD325) Antikörper

Antigen: N-Cadherin (CD325)

Klonalität: Monoklonal (6G11 1)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (IHC), Immunhistochemie (IHC)

Zertifikate: ISO 9001:2008, ISO 13485:2003

Anbieter: Dako

Produktnummer: ABIN370658

Produktbeschreibung

Weitere Bezeichnung: N-Cadherin

Immunogen: Synthetic peptide corresponding to human N-cadherin amino acids 878-905 1

Isotyp: IgG1, kappa chain  (Passende Sekundärantikörper)

Klon: 6G11 1

Beschreibung: Nerve cadherin, Neural-type cadherin (NCAD) Summary and explanation N-cadherin is a 140 kD protein that belongs to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. N-cadherin’s function is dependent on its association with the actin-cytoskeleton, which is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin. 5, 6 Expression of N-cadherin has been reported on a variety of normal tissues including neuronal, endothelial and muscle cells and a subpopulation of early hematopoietic progenitor cells. 7 Among neoplastic tissues, N-cadherin expression has been demonstrated in malignant noncarcinomatous neoplasms including mesotheliomas, 2-4, 8 chordomas, synovial sarcomas, malignant melanomas, epitheliod sarcomas, epitheliod angiosarcomas and clear cell sarcomas. 8 Serous and endometrioid tumors of the ovary have been demonstrated to be N-cadherin positive whereas mucinous tumors are negative. 9 Other N-cadherin positive neoplasms include renal cell carcinomas and some variant breast tumors including medullary breast carcinomas and sarcomatoid metaplastic breast carcinomas. 10, 11

Anwendungen

Applikationshinweise: This antibody is intended for laboratory use to identify qualitatively by light microscopy N-cadherin expressing cells in normal and neoplastic tissues using immunohistochemical (IHC) test methods. Positive results aid in the classification of mesotheliomas. 2-4 Differential identification is aided by the results from a panel of antibodies. Interpretation must be made within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist. Paraffin Sections Anti-N-Cadherin can be used on formalin-fixed, paraffin-embedded tissue sections. Pretreatment of tissue with proteolytic enzymes is not recommended. The deparaffinized tissue sections must be treated with heat prior to the IHC staining procedure. Heat-induced epitope retrieval (HIER) involves immersion of tissue sections in a pre-heated buffer solution and maintaining heat, either in a water bath or steamer (95-99 °C), or a pressure cooker (121 °C). For greater adherence of tissue sections to glass slides, the use of Silanized Slides (code S3003) is recommended. Target Retrieval Solution (code S1700) or 10x Concentrate (code S1699) is recommended using a 40-minute heating protocol for HIER performed at 95-99 °C. After thermal treatment, allow the jar with buffer and slides to cool for 20 minutes at room temperature. Rinse well with buffer or deionized water. Optimal HIER incubation times at temperatures greater than 99 °C should be determined by each individual laboratory. Cryostat Sections And Cell Smears Anti-N-Cadherin can be used for labelling acetone-fixed cryostat sections or fixed cell smears. Prior to IHC staining, tissues must be fixed and processed. Fixation prevents autolysis and putrefaction of excised tissues, preserves antigenicity, enhances the refractive index of tissue constituents and increases the resistance of cellular elements to tissue processing. Tissue processing includes dehydration, clearing of dehydrating agents, infiltration of embedding media, embedding and sectioning of tissues. The most common fixatives for IHC tissue preparations are discussed in the General Instructions for Immunohistochemical Staining. These are guidelines only. Optimal procedures must be determined and verified by the user. Follow the recommended procedure for the detection system selected. Staining interpretation The cellular staining pattern for anti-N-cadherin is membrane and cytoplasmic. Normal tissues 12 Anti-N-cadherin strongly immunostains ganglion cells and nerve in many normal tissue types. Positively Staining Tissue Elements Adrenal (3) 3/3 membrane and cytoplasm of adrenal cortical and adrenal medulla cells Brain, cerebellum (3) 3/3 diffuse staining of neuropil and membrane of Purkinje cells Brain , cerebrum (3) 3/3 diffuse staining of neuropil Stomach (3) 3/3 membrane and cytoplasm of chief, parietal and oxyphil cells Endometrium (3) 3/3 membrane and cytoplasm of endometrial glands and cytoplasm of endometrial stromal cells Kidney (3) 3/3 membrane and cytoplasm of renal tubule epithelium Ovary (3) 1/3 membrane and cytoplasm of surface epithelial cells and inclusion cysts epithelium Pancreas (3) 3/3 membrane and cytoplasm of acinar cells Pituitary (3) 3/3 diffuse staining of cell processes in neurohypophysis and membrane and cytoplasm of pituicytes in adenohypophysis Heart (3) 3/3 cell junction and cytoplasm of 100% of myocytes Liver (3) 3/3 membrane and cytoplasm of hepatocytes and membrane of bile duct epithelium Spleen (3) 3/3 membrane and cytoplasm of splenic sinuses Testis (3) 3/3 membrane and cytoplasm of 100% of sertoli cells, spermatogonia and seminiferous tubules Tonsil (3) 3/3 cytoplasm germinal center cells Parathyroid (3) 3/3 membrane and cytoplasm of chief cells Skeletal Muscle (3) 0/3 Colon (3) 0/3 Esophagus (3) 0/3 Lung (3) 0/3 Skin (3) 0/3 Bone Marrow (3) 0/3 Breast (3) 1/3 membrane of ductal epithelial cells (1 duct) Cervix (3) 0/3 Prostate (3) 0/3 Thymus (3) 0/3 Mesothelial Cells (3) 0/3 Nerve, peripheral (3) 0/3 (105521-002) 305844EFG_001 p. 3/7 Salivary Gland (3) 0/3 Small Intestine (3) 0/3 Thyroid (3) 0/3.

Buffer: Anti-N-cadherin is provided as tissue culture supernatant (containing fetal bovine serum) dialyzed against 0.05 mol/L Tris-HCl, pH 7.2, and 0.015 mol/L sodium azide. Contains stabilizing protein.

Lagerung: Store at 2-8 °C. Precautions: 1. For professional users. 2. This product contains sodium azide (NaN 3 ), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, NaN 3 may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used. 4. Wear appropriate Personal Protective Equipment to avoid contact with eyes and skin. 5. Unused reagents should be disposed of according to local, State, and Federal regulations. (105521-002) 305844EFG_001 p. 2/7

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.