Androgen Receptor (AR) Antikörper

Antigen: Androgen Receptor (AR)

Synonyme: KD, AIS, TFM, DHTR, SBMA, HYSP1, NR3C4, SMAX1, HUMARA, Tfm, AW320017, Andr, AR, DKFZp468G2216

Klonalität: Monoklonal (AR441)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p))

Zertifikate: ISO 9001:2008, ISO 13485:2003

Anbieter: Dako

Produktnummer: ABIN370645

Produktbeschreibung

Weitere Bezeichnung: Androgen Receptor

Immunogen: Synthetic peptide corresponding to amino acids 229…315 of the human androgen receptor coupled to keyhole limpet hemocyanin1

Isotyp: IgG1, kappa chain  (Passende Sekundärantikörper)

Klon: AR441

Beschreibung: Androgen receptor is an intracellular protein which belongs to a large family of hormone-induced transcription factors. 2,3 Androgen receptor mediates the biological actions of physiological androgens such as testosterone and 5 alpha-dihydrotestosterone, which are essential for differentiation, development and maintenance of the male reproductive organs. Complexes of androgen and receptor regulate growth responses in accessory sex organs by modulating specific gene transcription. 3

Spezifität: Monoclonal mouse anti-human androgen receptor, clone AR441 (anti-androgen receptor) specifically immunostains the nuclei of positive cells in human tissues. In Western blot analysis, the antibody identifies a 110 and 112 kD doublet in extracts of the metastatic prostate cancer cell line LNCap and in extracts of cells transfected with the gene for androgen receptor. 1 The antibody is unreactive with mouse androgen receptor in immunoblots of prostate extracts. Anti-androgen receptor is capable of recognizing and forming complexes with native androgen bound receptor. 1

Anwendungen

Applikationshinweise: Paraffin Sections Anti-androgen receptor can be used on formalin-fixed, paraffin-embedded tissue sections. The deparaffinized tissue sections must be treated with heat prior to the IHC staining procedure. For greater adherence of tissue sections to glass slides, the use of Silanized Slides (code S3003) is recommended. When using the water bath method, preheat a Coplin jar containing Target Retrieval Solution, High pH (code S3307 or S3308), as well as a water bath to 95-99 °C. When the temperature has stabilized, place tissue sections in the Coplin jar containing the preheated buffer. Heat the tissue sections for 20 minutes. After thermal treatment, allow the jar with buffer and slides to cool for 20 minutes at room temperature. Rinse well with distilled water and place slides in buffer. (115058-001) 303346EFG_001 p. 2/5 Cryostat Sections and Cell Smears Anti-androgen receptor can be used for labeling acetone-fixed cryostat sections or fixed cell smears. Follow the recommended procedure for the detection system selected. Staining interpretation The cellular staining pattern for anti-androgen is nuclear. Normal Cells Androgen receptor has been localized by immunohistochemistry (IHC) to the nuclei of a variety of normal cells. In the prostate, nuclear immunoreactivity has been observed in the secretory cells, basal cells, smooth muscle cells and fibroblasts. Moderate immunostaining has been found in spermatogonia, spermatocytes and Sertoli cells of the testes, with weaker staining seen in Leydig and peritubular myoid cells. Strong to moderate expression of androgen receptor has been demonstrated by IHC in uterine myometrial cells, cervical squamous cells, ovarian stromal cells, and syncytiotrophoblasts and cytotrophoblasts of the placenta. 4 Endometrial glandular and stromal cells also express androgen receptor at all phases of the menstrual cycle. 4,5 In the breast, the nuclei of acinar cells, myoepithelial cells and stromal cells were reported to stain positively. Androgen receptor was also demonstrated in cardiac muscle, striated muscle and in smooth muscle of arteries, bladder and GI tract. In skin, positive staining has been observed in cells of the hair follicles, sweat glands and squamous cells of the epidermis. Other cells which have been shown to give moderate to strong immunostaining include hepatocytes of the liver and cells of the anterior and posterior pituitary. 4 Tumor Cells In adenocarcinomas of the prostate androgen receptor has been demonstrated by IHC to be present in the nuclei of most neoplastic cells with neuroendocrine differentiation. 6 The majority of breast and ovarian carcinomas evaluated also express androgen receptor, as determined by IHC and biochemical assays. 7-10 The nuclei of glandular and stromal endometrial cells have been shown to stain positively in adenomyosis and external endometriosis. Malignant cells in both glandular and solid lesions of grade II endometrial adenocarcinomas were found to be immunoreactive. 5 Androgen receptor expression has also been demonstrated in meningiomas by Northern blot analysis and immunohistochemistry.

Buffer: Anti-androgen receptor is a mouse monoclonal antibody supplied in liquid form as tissue culture supernatant (containing fetal bovine serum) dialyzed against 0.05 mol/L Tris-HCl, pH 7.2, and 0.015 mol/L sodium azide.

Lagerung: Store at 2-8 °C. Precautions: 1. For professional users. 2. This product contains sodium azide (NaN 3 ), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, NaN 3 may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used. 4. Wear appropriate Personal Protective Equipment to avoid contact with eyes and skin. 5. Unused reagents should be disposed of according to local, State, and Federal regulations.

Forschungsgebiet: Hormone, Rezeptoren

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Publikationen

Publikationen: Johannesson, Sahlin, Masironi et al.: "Steroid receptor expression and morphology in provoked vestibulodynia." in: American journal of obstetrics and gynecology, Vol. 198, Issue 3, pp. 311.e1-6, 2008 (PubMed).

Voelter, Kleinsasser, Joa et al.: "Detection of hormone receptors in the human vocal fold." in: European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery, Vol. 265, Issue 10, pp. 1239-44, 2008 (PubMed).

Hofling, Ma, Sahlin et al.: "Expression of the androgen receptor and syndecan-1 in breast tissue during different hormonal treatments in cynomolgus monkeys." in: Climacteric : the journal of the International Menopause Society, Vol. 12, Issue 1, pp. 72-9, 2008 (PubMed).

Agrawal, Jele?, Grzebieniak et al.: "Androgen receptors as a prognostic and predictive factor in breast cancer." in: Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society, Vol. 46, Issue 3, pp. 269-76, 2008 (PubMed).