Caldesmon 1 (CALD1) Antikörper

Antigen: Caldesmon 1 (CALD1)

Synonyme: CDM, H-CAD, L-CAD, NAG22, MGC21352, AI195384, AV071549, AW536160, MGC30319, 4833423D12Rik, C920027I18Rik, CALD1, caldesmon, zgc:56389, si:bz30i22.4, si:rp71-30i22.4, DKFZp459K021

Klonalität: Monoklonal (H-CD1)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p))

Zertifikate: ISO 9001:2008, ISO 13485:2003

Anbieter: Dako

Produktnummer: ABIN370641

Produktbeschreibung

Weitere Bezeichnung: Caldesmon

Immunogen: Crude human uterus extract

Isotyp: IgG1, kappa chain  (Passende Sekundärantikörper)

Klon: H-CD1

Beschreibung: Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction. 2,3

Spezifität: Two closely related variants of human caldesmon have been identified which differ in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120–150 kD) is predominantly expressed in smooth muscle whereas I-caldesmon (70–80 kD) is found in non-muscle tissue and cells. Neither of the two variants have been detected in skeletal muscle. 2 Monoclonal anti-caldesmon, h-CD, recognizes only the 150 kD variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. 1

Anwendungen

Applikationshinweise: Paraffin Sections Anti-caldesmon, h-CD can be used on formalin-fixed, paraffin-embedded tissue sections. Prior to the IHC staining procedure, the deparaffinized tissue sections must be treated with a proteolytic enzyme followed by target retrieval. For greater adherence of tissue sections to glass slides, the use of Silanized Slides (code S3003) is recommended. Deparaffinized tissue sections must first be treated for 5 to 10 minutes with a mild enzyme solution. A recommended proteolytic enzyme is Proteinase K (code S3004) which must be further diluted 1:500 in 0.05 mol/L Tris-HCl, pH 7.6 to give a final concentration of 0.04 mg/mL. (112091-002) 303342EFG_001 p. 2/5 Following proteolytic digestion, tissue sections must be treated with heat. When using the water bath method, preheat a Coplin jar containing 0.01 mol/L citrate buffer, pH 6.0 as well as a water bath to 95-99 °C. When the temperature has stabilized, place tissue sections into the Coplin jar containing the preheated buffer. Heat the tissue sections for 40 minutes. For improved staining results and a shorter incubation time, Target Retrieval Solution (code S1700) can be used in place of the 0.01 mol/L citrate buffer. Under these conditions the incubation time in the water bath may be reduced to 20 minutes. After thermal treatment, allow the jar with buffer and slides to cool for 20 minutes at room temperature. Rinse well with distilled water and place slides into buffer. Cryostat Sections and Cell Smears Anti-caldesmon, h-CD can also be used to label cryostat sections or cell smears. Follow the recommended procedure for the detection system selected. Staining interpretation The staining pattern for anti-caldesmon is cytoplasmic. Normal Cells Monoclonal anti-caldesmon was found to localize h-caldesmon in cryostat sections of human tissues including developing visceral smooth muscle from 10-20 week-old fetal trachea, esophagus, jejunum and uterus. Aortic smooth muscle cells from 10 and 20 week-old fetuses were unreactive with anti-caldesmon. 1 In the adult, caldesmon expression was demonstrated in vascular and visceral smooth muscle cells but not epithelial cells, endothelial cells or connective tissue fibroblasts. Cells of the tunica media and a subpopulation of smooth muscle cells of the sub-endothelial intima of the adult aorta were shown to stain positively with anti-caldesmon. 1 In immunohistochemical (IHC) studies on cryostat sections of normal human breast, monoclonal anti-caldesmon labeled the smooth muscle cells of blood vessels and a subset of myoepithelial cells in the galactophorous sinuses. Myoepithelial cells of the lobules, ducts and luminal epithelial cells of normal breast did not react positively. 4 Tumor Cells In IHC studies on breast carcinoma, monoclonal anti-caldesmon has been demonstrated to label a subpopulation of myoepithelial cells but is unreactive with myofibroblasts and tumor cells.

Buffer: Anti-human caldesmon, h-CD is a mouse monoclonal antibody supplied in liquid form as tissue culture supernatant (containing fetal bovine serum) dialyzed against 0.05 mol/L Tris-HCl, pH 7.2 and 0.015 mol/L sodium azide.

Lagerung: Store at 2-8 °C. Precautions: 1. For professional users. 2. This product contains sodium azide (NaN 3 ), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, NaN 3 may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used. 4. Wear appropriate Personal Protective Equipment to avoid contact with eyes and skin. 5. Unused reagents should be disposed of according to local, State, and Federal regulations.

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Publikationen

Publikationen: Puerot, Derrue, Coindre et al.: "Strong smooth muscle differentiation is dependent on myocardin gene amplification in most human retroperitoneal leiomyosarcomas." in: Cancer research, Vol. 69, Issue 6, pp. 2269-78, 2009 (PubMed).

Matsubara, Morikawa, Goto et al.: "Subepithelial myofibroblast in lung adenocarcinoma: a histological indicator of excellent prognosis." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 22, Issue 6, pp. 776-85, 2009 (PubMed).

Takacs, Gualtieri, Nassiri et al.: "Caldesmon expression is decreased in women with anterior vaginal wall prolapse: a pilot study." in: International urogynecology journal and pelvic floor dysfunction, Vol. 20, Issue 8, pp. 985-90, 2009 (PubMed).

Hansen, Suorensen, Spindler et al.: "Microvessel density and the association with single nucleotide polymorphisms of the vascular endothelial growth factor receptor 2 in patients with colorectal cancer." in: Virchows Archiv : an international journal of pathology, Vol. 456, Issue 3, pp. 251-60, 2010 (PubMed).

Takacs, Zhang, Jaramillo et al.: "The effects of estrogen, progesterone and polypropylene mesh on vaginal smooth muscle cell proliferation." in: Journal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi, Vol. 46, Issue 1, pp. 9-15, 2010 (PubMed).