Amyloid beta (Abeta) Antikörper

Antigen: Amyloid beta (Abeta)

Synonyme: AAA, AD1, PN2, ABPP, APPI, CVAP, ABETA, CTFgamma, aaa, ad1, pn2, abpp, appi, cvap, abeta, MGC88882, ctfgamma, APP

Klonalität: Monoklonal (6F-3D)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p))

Zertifikate: ISO 9001:2008, ISO 13485:2003

Anbieter: Dako

Produktnummer: ABIN370614

Produktbeschreibung

Weitere Bezeichnung: Beta-Amyloid

Immunogen: A synthetic peptide consisting of residues 8-17 (ser-gly-tyr-glu-val-his-his-gln-lys-leu) with an additional C-terminal cysteine coupled to keyhole limpet hemocyanin via the cysteine residue.

Isotyp: IgG1, kappa chain  (Passende Sekundärantikörper)

Klon: 6F-3D

Beschreibung: Synonyms for antigen Amyloid beta-protein (A beta P), Amyloid beta-peptide, Abeta, betaA4 protein. The gene encoding beta-amyloid protein is located on chromosome 21 (2). The beta-amyloid protein is a 39-43 residue fragment of a much larger molecule, the beta-amyloid precursor protein, and has variable N- or C-termini (3-5). Beta-amyloid is present in senile plaques, neurofibrillary tangles and cerebrovascular deposits in patients with Alzheimer’s disease and Down’s syndrome (trisomy 21) (4, 6). Alzheimer’s disease is the most common cause of dementia among elderly people. The pathological hallmarks of Alzheimer’s diseased brains include cortical atrophy, extensive neuronal loss and intracellular and extracellular abnormal fibrillar deposits, i.e. neurofibrillary tangles and senile plaques, which are characterized by a heavy accumulation of beta-amyloid. However, beta-amyloid is also produced in small quantities under physiological conditions of normal brain (4, 5).

Spezifität: In immunocytochemistry, the antibody labels beta-amyloid containing the N-terminal epitope (1, 5). In ELISA, the antibody reacts with a synthetic peptide corresponding to amino acids 9-14 of beta-amyloid (7). As demonstrated by immunocytochemistry, the antibody cross-reacts with the beta-amyloid-equivalent protein in dogs (8).

Anwendungen

Applikationshinweise: Paraffin sections: The antibody can be used for labelling paraffin-embedded tissue sections fixed in formalin. Pre-treatment of tissues with 85%-100% formic acid or heat-induced epitope retrieval is required. For heat-induced epitope retrieval, optimal results are obtained with DakoCytomation Target Retrieval Solution, code No. S 1700, DakoCytomation Target Retrieval Solution, pH 9.0, code No. S 2368, DakoCytomation Target Retrieval Solution, High pH, code No. S 3308, or 10 mmol/L citrate buffer, pH 6.0. Pre-treatment of tissues with DakoCytomation Proteinase K, code No. S 3020, was found inefficient. The tissue sections should not dry out during the treatment or during the following immunocytochemical staining procedure. Frozen sections and cell preparations: The antibody cannot be used for labelling frozen sections. Dilution: Monoclonal Mouse Anti-Human Beta-Amyloid, code No. M 0872, may be used at a dilution range of 1:50-1:100 when applied on formalin-fixed, paraffin-embedded sections of human brain from a patient with Alzheimer's disease and using 2-10 minutes epitope retrieval in 85%-100% formic acid or 20 minutes heat-induced epitope retrieval in DakoCytomation Target Retrieval Solution, code No. S 1700, and 30 minutes incubation at room temperature with the primary antibody. Optimal conditions may vary depending on specimen and preparation method, and should be determined by each individual laboratory. The recommended negative control is DakoCytomation Mouse IgG1, code No. X 0931, diluted to the same mouse IgG concentration as the primary antibody. Unless the stability of the diluted antibody and negative control has been established in the actual staining procedure, it is recommended to dilute these reagents immediately before use, or dilute in DakoCytomation Antibody Diluent, code No. S 0809. Positive and negative controls should be run simultaneously with patient specimen. Abnormal tissues: The antibody labels senile plaques and congofile deposits in vessel walls in brains of patients with Alzheimer's disease, Down's syndrome and congofil angiopathy.

Buffer: Monoclonal mouse antibody provided in liquid form as cell culture supernatant dialysed against 50 mmol/L Tris/HCl, pH 7.2, and containing 15 mmol/L NaN 3 .

Lagerung: Store at 2-8 °C. Precautions: 1. For professional users. 2. This product contains sodium azide (NaN 3 ), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.

Forschungsgebiet: Neurologie, Alzheimer-Krankheit (Morbus Alzheimer)

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Publikationen

Publikationen: Koprowski, Steplewski, Mitchell et al.: "Colorectal carcinoma antigens detected by hybridoma antibodies." in: Somatic cell genetics, Vol. 5, Issue 6, pp. 957-71, 1980 (PubMed).

Hanai, Shitara, Furuya et al.: "Detailed characterization of reactivities of anti-gastric cancer monoclonal antibodies to carbohydrate antigen." in: Anticancer research, Vol. 10, Issue 6, pp. 1579-86, 1991 (PubMed).

Koprowski, Brockhaus, Blaszczyk et al.: "Lewis blood-type may affect the incidence of gastrointestinal cancer." in: Lancet, Vol. 1, Issue 8285, pp. 1332-3, 1982 (PubMed).

Arends, Verstynen, Bosman et al.: "Distribution of monoclonal antibody-defined monosialoganglioside in normal and cancerous human tissues: an immunoperoxidase study." in: Hybridoma, Vol. 2, Issue 2, pp. 219-29, 1984 (PubMed).

Atkinson, Ernst, Herlyn et al.: "Gastrointestinal cancer-associated antigen in immunoperoxidase assay." in: Cancer research, Vol. 42, Issue 11, pp. 4820-3, 1982 (PubMed).

Magnani, Nilsson, Brockhaus et al.: "A monoclonal antibody-defined antigen associated with gastrointestinal cancer is a ganglioside containing sialylated lacto-N-fucopentaose II." in: The Journal of biological chemistry, Vol. 257, Issue 23, pp. 14365-9, 1983 (PubMed).

Byrne, Ross, Faull et al.: "High-throughput quantification of Alzheimer's disease pathological markers in the post-mortem human brain." in: Journal of neuroscience methods, Vol. 176, Issue 2, pp. 298-309, 2009 (PubMed).

Herring, Ambruee, Tomm et al.: "Environmental enrichment enhances cellular plasticity in transgenic mice with Alzheimer-like pathology." in: Experimental neurology, Vol. 216, Issue 1, pp. 184-92, 2009 (PubMed).

Reitz, Honig, Vonsattel et al.: "Memory performance is related to amyloid and tau pathology in the hippocampus." in: Journal of neurology, neurosurgery, and psychiatry, Vol. 80, Issue 7, pp. 715-21, 2009 (PubMed).

Schwab, Arai, Hasegawa et al.: "TDP-43 pathology in familial British dementia." in: Acta neuropathologica, Vol. 118, Issue 2, pp. 303-11, 2009 (PubMed).

Giuliani, Vernay, Leuba et al.: "Decreased behavioral impairments in an Alzheimer mice model by interfering with TNF-alpha metabolism." in: Brain research bulletin, Vol. 80, Issue 4-5, pp. 302-8, 2009 (PubMed).

Capucchio, Muarquez, Pregel et al.: "Parenchymal and vascular lesions in ageing equine brains: histological and immunohistochemical studies." in: Journal of comparative pathology, Vol. 142, Issue 1, pp. 61-73, 2009 (PubMed).

Rauramaa, Pikkarainen, Englund et al.: "Cardiovascular diseases and hippocampal infarcts." in: Hippocampus, 2010 (PubMed).