Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) Antikörper

Antigen: Actin, alpha 2, Smooth Muscle, Aorta (ACTA2)

Synonyme: AAT6, ACTSA, Actvs, a-SMA, SMalphaA, 0610041G09Rik, wu:fb63d03, MGC78870, ACTA2, actc1, actsa, MGC89192, MGC127800, ACT-4, DKFZp469E0314, Acta2, asma, acta, cftd, mpfd, nem1, nem2, nem3, acta2, cftd1, cftdm, MGC52636, MGC79039

Klonalität: Monoklonal (1A4)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p))

Zertifikate: ISO 9001:2008, ISO 13485:2003

Anbieter: Dako

Produktnummer: ABIN370610

Produktbeschreibung

Weitere Bezeichnung: Smooth Muscle Actin

Immunogen: N-terminal synthetic decapeptide of alpha -smooth muscle actin coupled to keyhole limpet haemocyanin (KLH) (4).

Isotyp: IgG2a, kappa chain  (Passende Sekundärantikörper)

Klon: 1A4

Beschreibung: Cytoplasmic actins, which belong to the microfilament system of cytoskeleton proteins, are some of the most conserved eukaryotic proteins being expressed in mammals and birds. The actin protein consists of six isoforms, varying in their amino acid sequence, but all having the same molecular mass of 42 kDa. The isoforms show more than 90% overall sequence homology, but only 50-60% homology in their 18 N-terminal residues. The N-terminal region appears to be a major antigenic region (4). There are different alpha isoforms specific for muscle tissues, i.e. skeletal muscle alpha , cardiac muscle alpha , and smooth muscle alpha , respectively (1). The beta - and gamma -actins may be present in muscle cells as well as most other cell types in the body, including non-muscle cells (5).

Spezifität: In Western blotting and SDS-PAGE immunoblotting of the alpha -smooth muscle isoform of actin, the antibody labels a band corresponding to alpha -smooth muscle actin (4). As demonstrated by Western blotting and/or immunocytochemistry, the antibody cross-reacts with the alpha -smooth muscle actin-equivalent protein in chicken, cow and rat (4).

Synonyme: AAT6, ACTSA, Actvs, a-SMA, SMalphaA, 0610041G09Rik, wu:fb63d03, MGC78870, ACTA2, actc1, actsa, MGC89192, MGC127800, ACT-4, DKFZp469E0314, Acta2, asma, acta, cftd, mpfd, nem1, nem2, nem3, acta2, cftd1, cftdm, MGC52636, MGC79039

Anwendungen

Applikationshinweise: Paraffin sections: The antibody can be used for labelling paraffin-embedded tissue sections fixed in formalin. Pre-treatment of tissues with heat-induced epitope retrieval is recommended. Optimal results are obtained with 10 mmol/L Tris buffer, 1 mmol/L EDTA, pH 9.0. Less optimal results are obtained with Dako Target Retrieval Solution, High pH, code S3308, or 10 mmol/L citrate buffer, pH 6.0. However, Dako Target Retrieval Solution, code S1700 was found inefficient. Pre-treatment of tissues with proteinase K was found destructive of the epitope. The tissue sections should not dry out during the treatment or during the following immunohistochemical staining procedure. Frozen sections and cell preparations: The antibody can be used for labelling acetone-fixed, frozen sections (1). Dilution: Monoclonal Mouse Anti-Human Smooth Muscle Actin, code M0851, may be used at a dilution range of 1:50-1:100 when applied on formalin-fixed, paraffin-embedded sections of normal human colon and using 20 minutes heat-induced epitope retrieval in 10 mmol/L Tris buffer, 1 mmol/L EDTA, pH 9.0, and 30 minutes incubation at room temperature with the primary antibody. Optimal conditions may vary depending on specimen and preparation method, and should be determined by each individual laboratory. The recommended negative control is Dako Mouse IgG2a, code X0943, diluted to the same mouse IgG concentration as the primary antibody. Unless the stability of the diluted antibody and negative control has been established in the actual staining procedure, it is recommended to dilute these reagents immediately before use, or dilute in Dako Antibody Diluent, code S0809. Positive and negative controls should be run simultaneously with patient specimen. Visualization: Dako LSAB/HRP kit, code K0679, and Dako EnVision/HRP kits, codes K4004 and K4006, are recommended. For frozen sections and cell preparations, the Dako APAAP kit, code K0670, is a good alternative if endogenous peroxidase staining is a concern. Follow the procedure enclosed with the selected visualization kit. Automation: The antibody is well-suited for immunohistochemical staining using automated platforms, such as the Dako Autostainer. Performance characteristics: Cells labelled by the antibody display a cytoplasmic staining pattern. Normal tissues: The antibody labels smooth muscle cells in blood vessels and, additionally, salivary ducts and myoepithelial cells around acini in salivary glands (3). Smooth muscle cells in 35/36 normal uterine myometria were also positively labelled (2). Further, a temporal labelling of perisinusoidal liver cells has been observed (6). In frozen tissues, the antibody labels myofibroblasts and myoepithelial cells around acini and ducts of the breast, whereas epithelia (adeno, squamous), lymphocytes, cardiac- and skeletal muscle cells, endothelial cells, fat cells, Schwann cells and fibroblast are negative (1).

Buffer: Monoclonal mouse antibody provided in liquid form as cell culture supernatant dialysed against 0.05 mol/L Tris/HCl, pH 7.2, and containing 15 mmol/L NaN 3 .

Lagerung: Store at 2-8 °C. Precautions: 1. For professional users. 2. This product contains sodium azide (NaN 3 ), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used. 4. Wear appropriate Personal Protective Equipment to avoid contact with eyes and skin. 5. Unused solution should be disposed of according to local, State and Federal regulations.

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Bilder

Uterine leiomyoma. The majority of the neoplastic cells show amoderate to strong cytoplasmic staining reaction.

Leiomyosarcoma. The majority of the neoplastic cells show amoderate to strong cytoplasmic staining reaction.

Colon. The smooth muscle cells show a moderate to strongcytoplasmic staining reaction.