CD61 Platelet Glycoprotein IIIa Antikörper

Antigen: CD61 Platelet Glycoprotein IIIa

Klonalität: Monoklonal (Y2-51)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p))

Zertifikate: ISO 9001:2008, ISO 13485:2003

Anbieter: Dako

Produktnummer: ABIN370576

Produktbeschreibung

Weitere Bezeichnung: CD61, Platelet Glycoprotein IIIa

Immunogen: Phytohemagglutinin (PHA)-stimulated peripheral blood cells (4).

Isotyp: IgG1, kappa chain  (Passende Sekundärantikörper)

Klon: Y2-51

Beschreibung: Synonyms for antigen CD61A, GPllb/llla, beta3 integrin chain, beta-3 integrin (1). CD61 has a molecular mass of approximately 110 KDa and is the common beta-subunit in the CD41/CD61 and in the CD51/CD61 complexes, both of which are noncovalently associated heterodimers. The complexes require divalent cations for complex integrity and receptor functions (1). The CD41/CD61 complex appears early in megakaryocyte maturation (2). The activated CD41/CD61 complex is a receptor for von Willebrand factor, soluble fibrinogen and fibronectin (1) and plays a central role in platelet activation and aggregation (3). The CD51/CD61 plays a role in tumour metastasis and adenoviral infection (1).

Spezifität: Anti-CD61, Y2/51, was included in the Fourth International Workshop and Conference on Human Leucocyte Differentitation Antigens and studies by a number of laboratories confirmed its reactivity with CD61 (5). Anti-CD61, Y2/51, labels platelets in peripheral blood and bone marrow and it also reacts with megakaryocytes (4).

Anwendungen

Applikationshinweise: Paraffin sections: The antibody can be used for labelling paraffin-embedded tissue sections fixed in formalin. Pre-treatment of tissues with heat-induced epitope retrieval is recommended. Optimal results are obtained with, DakoCytomation Target Retrieval Solution, code No. S 1700, or 10 mmol/L citrate buffer, pH 6.0 and pre-treatment of tissues with proteinase K. The tissue sections should not dry out during the treatment or during the following immunocytochemical staining procedure. Frozen sections and cell preparations: The antibody can be used for labelling frozen sections, fixed cell smears and fixed cytospins. Dilution: Monoclonal Mouse Anti-CD61, code No. M 0753, may be used at a dilution range of 1:50-1:100, in the DAKO EnVision system when applied on formalin-fixed, paraffin-embedded sections of human tonsil and using 20 minutes heat-induced epitope retrieval in DakoCytomation Target Retrieval solution, code No. S 1700, and 30 minutes incubation at room temperature with the primary antibody. Monoclonal Mouse Anti-CD61, code No. M 0753, may be used at a dilution range of 1:50-1:100, in the APAAP technique, when applied on frozen sections, fixed cell smears and fixed cytospins, using 30 minutes incubation at room temperature with the primary antibody. Optimal conditions may vary depending on specimen and preparation method, and should be determined by each individual laboratory. The recommended negative control is DakoCytomation Mouse IgG1, code No. X 0931, diluted to the same mouse IgG concentration as the primary antibody. Unless the stability of the diluted antibody and negative control has been established in the actual staining procedure, it is recommended to dilute these reagents immediately before use, or dilute in DakoCytomation Antibody Diluent, code No. S 0809. Positive and negative controls should be run simultaneously with patient specimen. Visualization: DAKO LSAB/HRP kit, code No. K 0679, and DAKO EnVision/HRP kits, code Nos. K 4004 and K 4006, are recommended. For frozen sections and cell preparations, the DakoCytomation APAAP kit, code No. K 0670, is a good alternative if endogenous peroxidase staining is a concern. Follow the procedure enclosed with the selected visualization kit. Automation: The antibody is well-suited for immunocytochemical staining using automated platforms, such as the DakoCytomation Autostainer. Performance characteristics: Cells labelled by the antibody display staining confined to the cell surface membrane. In peripheral human lymphoid tissue and in normal human tonsil, the antibody shows labelling pattern corresponding to reactivity restricted to platelets.

Buffer: Monoclonal mouse antibody provided in liquid form as cell culture supernatant dialysed against 0.05 mol/L Tris/HCl, pH 7.2, and containing 15 mmol/L NaN 3 ,

Lagerung: Store at 2-8 °C. Precautions: 1. For professional users. 2. This product contains sodium azide (NaN 3 ), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Publikationen

Publikationen: Sarfati, Shaw, Simmonds: "Commentary: Inequalities in cancer screening programmes." in: International journal of epidemiology, 2010 (PubMed).