Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) Antikörper

Antigen: Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8)

Synonyme: CD30, Ki-1, D1S166E, Ki, Cd30, RP23-306F22.1, TNFRSF8, TNFSF8

Klonalität: Monoklonal (Ber-H2)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p))

Zertifikate: ISO 9001:2008, ISO 13485:2003

Anbieter: Dako

Produktnummer: ABIN370573

Produktbeschreibung

Weitere Bezeichnung: CD30

Immunogen: Co cell line established from a patient with Hodgkin’s disease of T-cell lineage (1, 6).

Isotyp: IgG1, kappa chain  (Passende Sekundärantikörper)

Klon: Ber-H2

Beschreibung: Ki-1 antigen (1). CD30 is a transmembrane cytokine receptor belonging to the tumour necrosis factor (TNF) receptor superfamily. Mature CD30 has a molecular mass of 120 kDa and is derived from a 90 kDa precursor protein (2). The extracellular domain of CD30 is homologous to that of TNF receptor superfamily members, whereas there is no homology in the cytoplasmic domain, suggesting major differences in signalling mechanisms (3). The intracellular part of CD30 possesses kinase activity, indicating that CD30 plays a role in regulating the function, differentiation and/or proliferation of normal lymphoid cells (2). A soluble 85 kDa form of CD30, sCD30, released from the membrane-bound molecule by proteolytic cleavage, can be detected in the sera of patients with CD30-expressing neoplasms (3, 4). CD30 expression is found on Hodgkin and Reed-Sternberg (H-RS) cells, anaplastic large-cell lymphoma cells, and on activated B and T lymphocytes (2). In non-lymphoid tissues and neoplasms, CD30 expression has been confirmed in embryonal carcinomas, seminomas, decidual cells and mesotheliomas (5).

Spezifität: The antibody was clustered as anti-CD30 at the Fourth International Workshop and Conference on Human Leucocyte Differentiation Antigens held in Vienna in 1989 (7). SDS-PAGE analysis of immunoprecipitates formed between lysate of 125 I-labelled COS cells transfected with cDNA encoding CD30 and the antibody shows reaction with a 120 kDa protein corresponding to CD30. Mock-transfected COS cells were negative. The epitope recognized by the antibody is located between amino acid residues 112 and 412 (8). The antibody labels: Cell lines derived from Hodgkin’s disease, L428, L540, L591, Co, Ho and KM-H2, HTLV-1 transfected T-cell lines, Hut-102 and MT-2, EBV-transformed B-cell lines (non-Burkitt), B95-8 (monkey), BJA-B and Cess, and the myeloid cell line, K 562 (1).

Anwendungen

Applikationshinweise: Paraffin sections: The antibody can be used for labelling paraffin-embedded tissue sections fixed in formalin. Pre-treatment of tissues with heat-induced epitope retrieval is required. Optimal results are obtained with Dako Target Retrieval Solution, code S1700, or 10 mmol/L Tris buffer, 1 mmol/L EDTA, pH 9.0 Less optimal results are obtained with Dako Target Retrieval Solution, High pH, code S3308, or 10 mmol/L citrate buffer, pH 6.0. Pre-treatment of tissues with proteinase K was found inefficient. The tissue sections should not dry out during the treatment or during the following immunohistochemical staining procedure. Frozen sections and cell preparations: The antibody can be used for labelling acetone-fixed frozen sections and cytospin preparations (1, 5). Dilution: Monoclonal Mouse Anti-Human CD30, code M0751, may be used at a dilution range of 1:20-1:40 when applied on formalin-fixed, paraffin-embedded sections of Hodgkin's lymphomas or ALCL and using 20 minutes heat-induced epitope retrieval in Dako Target Retrieval Solution, code S1700, and 30 minutes incubation at room temperature with the primary antibody. Optimal conditions may vary depending on specimen and preparation method, and should be determined by each individual laboratory. The recommended negative control is Dako Mouse IgG1, code X0931, diluted to the same mouse IgG concentration as the primary antibody. Unless the stability of the diluted antibody and negative control has been established in the actual staining procedure, it is recommended to dilute these reagents immediately before use, or dilute in Dako Antibody Diluent, code S0809. Positive and negative controls should be run simultaneously with patient specimen. Visualization: Dako LSAB/HRP kit, code K0679, and Dako EnVision/HRP kits, codes. K4004 and K4006, are recommended. For frozen sections and cell preparations, the Dako APAAP kit, code K0670, is a good alternative if endogenous peroxidase staining is a concern. Follow the procedure enclosed with the selected visualization kit. Automation: The antibody is well suited for immunohistochemical staining using automated platforms, such as the Dako Autostainer. Product-specific limitations Cells labelled by the antibody display a membrane and/or a dot like cytoplasmic staining (1). A diffuse or finely granular cytoplasmic staining was observed in the endothelial cells in 21/33 cases of haemangioma, 4/10 cases of lymphangioma, 4/9 cases of mixed tumours with both components, and 6/10 cases of angioleiomyoma (9).

Buffer: Monoclonal mouse antibody provided in liquid form as cell culture supernatant dialysed against 0.05 mol/L Tris/HCl, pH 7.2, and containing 15 mmol/L NaN 3 .

Lagerung: Store at 2-8 °C. Precautions: 1. For professional users. 2. This product contains sodium azide (NaN 3 ), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used. 4. Wear appropriate Personal Protective Equipment to avoid contact with eyes and skin. 5. Unused solution should be disposed of according to local, State and Federal regulations.

Forschungsgebiet: CD Antigene, Oberflächenrezeptoren der Immunzellen

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Bilder

Classic Hodgkin lymphoma. The majority of theReed-Sternberg cells show a distinct membranous staining reactionand a paranuclear dot-like reaction.

Anaplastic large cell lymphoma. The majority of the neoplasticcells show a distinct membranous staining reaction.

Tonsil. Activated lymphocytes and plasma cells show amoderate to strong staining reaction.

Publikationen

Publikationen: Chang, Li, Smithson: "Immunocytochemical study of small round cell tumors in routinely processed specimens." in: Archives of pathology & laboratory medicine, Vol. 113, Issue 12, pp. 1343-8, 1990 (PubMed).

Van Muijen, Ruiter, Warnaar: "Coexpression of intermediate filament polypeptides in human fetal and adult tissues." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 57, Issue 4, pp. 359-69, 1987 (PubMed).

Hurlimann: "Desmin and neural marker expression in mesothelial cells and mesotheliomas." in: Human pathology, Vol. 25, Issue 8, pp. 753-7, 1994 (PubMed).

Pollock, Rampling, Greenwald et al.: "Desmin expression in rhabdomyosarcoma: influence of the desmin clone and immunohistochemical method." in: Journal of clinical pathology, Vol. 48, Issue 6, pp. 535-8, 1995 (PubMed).

Oliva, Young, Clement et al.: "Cellular benign mesenchymal tumors of the uterus. A comparative morphologic and immunohistochemical analysis of 33 highly cellular leiomyomas and six endometrial stromal nodules, two frequently confused tumors." in: The American journal of surgical pathology, Vol. 19, Issue 7, pp. 757-68, 1995 (PubMed).

Pitt, Roberts, Agbamu et al.: "The nature of atypical multinucleated stromal cells: a study of 37 cases from different sites." in: Histopathology, Vol. 23, Issue 2, pp. 137-45, 1993 (PubMed).

Miettinen, Sarlomo-Rikala, Sobin et al.: "Esophageal stromal tumors: a clinicopathologic, immunohistochemical, and molecular genetic study of 17 cases and comparison with esophageal leiomyomas and leiomyosarcomas." in: The American journal of surgical pathology, Vol. 24, Issue 2, pp. 211-22, 2000 (PubMed).

Goebel, Warlo: "Progress in desmin-related myopathies." in: Journal of child neurology, Vol. 15, Issue 9, pp. 565-72, 2001 (PubMed).

Eberle, Hanson, Killian et al.: "Immunoguided Laser Assisted Microdissection Techniques for DNA Methylation Analysis of Archival Tissue Specimens." in: The Journal of molecular diagnostics : JMD, 2010 (PubMed).