Kallikrein-Related Peptidase 3 (KLK3) Antikörper

Antigen: Kallikrein-Related Peptidase 3 (KLK3)

Synonyme: APS, PSA, hK3, KLK2A1, KLK3, KLKB1

Klonalität: Monoklonal (ER-PR8)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p))

Zertifikate: ISO 9001:2008, ISO 13485:2003

Anbieter: Dako

Produktnummer: ABIN370572

Produktbeschreibung

Weitere Bezeichnung: Prostate-Specific Antigen

Immunogen: Purified human prostate-specific antigen.

Isotyp: IgG1, kappa chain  (Passende Sekundärantikörper)

Klon: ER-PR8

Beschreibung: Kallikrein 3 (hK3) (3). Summary and explanation Prostate-Specific Antigen (PSA) is a 33 kDa protein belonging to the kallikrein family of proteases (4). It is primarily produced by the prostatic epithelium and the epithelial lining of the periurethal glands (3), and is secreted at concentrations of 0.5-2 g/L into the seminal fluid, where it liquefies the gel forming after ejaculation by digesting the major gel-forming proteins. Five to six isoenzymes of PSA can be separated from seminal fluid by isoelectric focusing and ion exchange chromatography (4). From the normal and hyperplastic prostate, PSA leaks into the extracellular fluid and diffuses into the blood, where the concentration is approximately one million-fold lower than in seminal fluid. In prostate cancer, the architecture and polarization of the epithelial cells are deranged, leading to active secretion into the extracellular space and into the circulation, and causing increased serum PSA concentrations (4). PSA is strongly expressed in both normal and neoplastic prostatic tissue. Although PSA can be considered prostate-specific, PSA and/or PSA gene expression has been detected at low levels in some extraprostatic tissues such as normal breast tissue (3, 4), breast tumours, breast milk, female serum, endometrium, adrenal neoplasms and renal cell carcinomas (3).

Spezifität: In Western blotting of PSA purified from seminal plasma the antibody labels bands corresponding to PSA under reducing conditions, whereas no labelling was observed under non-reducing conditions (5). Anti-PSA,

Anwendungen

Applikationshinweise: Paraffin sections: The antibody can be used for labelling paraffin-embedded tissue sections fixed in formalin. Pre-treatment of tissues with proteinase K or heat-induced epitope retrieval is recommended. For heat-induced epitope retrieval, optimal results are obtained with Dako Target Retrieval Solution, High pH, Dilution: Monoclonal Mouse Anti-Human PSA, The cellular staining pattern is cytoplasmic. Normal tissues: The antibody strongly labels prostatic epithelium. No labelling was observed in 34 other human tissues, including brain,skin, muscle, nerve, breast, lymph node, spleen, thymus, different parts of the respiratory, digestive, urinary and genital systems (8). Abnormal tissues: In benign hyperplastic prostate, the antibody shows clear staining in luminal (secretory) epithelial cells and no stromal reactivity (1). Of 60 patients with prostate cancer, 42 (70%) showed high and 18 (30%) low PSA expression with the antibody (2). No labelling is observed in adenocarcinoma of colon, bladder and adrenal cortex, in melanoma, hepatoblastoma, squamous cell carcinoma of skin and lung, and fibrosarcoma. The antibody has been reported to label weakly some collecting ducts and tubular cells in the kidney, ductal cells in the parotid gland and some pancreatic ductal cells (8).

Buffer: Monoclonal mouse antibody provided in liquid form as cell culture supernatant dialysed against 0.05 mol/L Tris/HCl, pH 7.2, and containing 15 mmol/L NaN 3 .

Lagerung: Store at 2-8 °C. Precautions: 1. For professional users. 2. This product contains sodium azide (NaN 3 ), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used. 4. Wear appropriate Personal Protective Equipment to avoid contact with eyes and skin. 5. Unused solution should be disposed of according to local, State and Federal regulations.

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Publikationen

Publikationen: Linke: "Monoclonal antibodies against amyloid fibril protein AA. Production, specificity, and use for immunohistochemical localization and classification of AA-type amyloidosis." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 32, Issue 3, pp. 322-8, 1984 (PubMed).

Linke: "Identification of amyloid protein AA with a monoclonal antibody." in: Blut, Vol. 45, Issue 6, pp. 407-9, 1983 (PubMed).

Linke, Gaertner, Michels: "High-sensitivity diagnosis of AA amyloidosis using Congo red and immunohistochemistry detects missed amyloid deposits." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 43, Issue 9, pp. 863-9, 1995 (PubMed).

Linke: "Highly sensitive diagnosis of amyloid and various amyloid syndromes using Congo red fluorescence." in: Virchows Archiv : an international journal of pathology, Vol. 436, Issue 5, pp. 439-48, 2000 (PubMed).

Sipe, Cohen: "Review: history of the amyloid fibril." in: Journal of structural biology, Vol. 130, Issue 2-3, pp. 88-98, 2000 (PubMed).