Bromodeoxyuridine (BrDU) Antikörper

Antigen: Bromodeoxyuridine (BrDU)

Klonalität: Monoklonal (Bu20a)

Wirt: Maus

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p))

Zertifikate: ISO 9001:2008, ISO 13485:2003

Anbieter: Dako

Produktnummer: ABIN370569

Produktbeschreibung

Weitere Bezeichnung: Bromodeoxyuridine

Immunogen: Bromodeoxyuridine conjugated to bovine serum albumin.

Isotyp: IgG1, kappa chain  (Passende Sekundärantikörper)

Klon: Bu20a

Beschreibung: Bromodeoxyuridine (BrdU) is a thymidine analogue, capable of being incorporated into DNA in place of thymidine during DNA synthesis. By pulse labelling with BrdU, the DNA synthesis in the cells can be analysed by detection with anti-BrdU antibodies. Antibodies against BrdU recognize BrdU in single-stranded DNA (produced by partial denaturation of double-stranded DNA), as well as free BrdU or BrdU coupled to a protein carrier (1).

Spezifität: Anti-BrdU,

Anwendungen

Applikationshinweise: Paraffin sections: The antibody can be used for labelling paraffin-embedded tissue sections fixed in formalin. Pre-treatment of tissues with heat-induced epitope retrieval is required. For heat-induced epitope retrieval, 10 mmol/L citrate buffer, pH 6.0 was found efficient. Pre-treatment of tissues with proteinase K should be omitted. The tissue sections should not dry out during the treatment or during the following immunocytochemical staining procedure. Frozen sections and cell preparations: The antibody is suitable for labelling acetone-fixed, frozen sections and cell preparations.

Buffer: Monoclonal mouse antibody provided in liquid form as cell culture supernatant dialysed against 0.05 mol/L Tris/HCl, pH 7.2, and containing 15 mmol/L NaN 3 .

Lagerung: Store at 2-8 °C. Precautions: 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains sodium azide (NaN 3 ), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Myskow, Krajewski, Salter et al.: "Paraffin section immunophenotyping of non-Hodgkin's lymphoma, using a panel of monoclonal antibodies." in: American journal of clinical pathology, Vol. 90, Issue 5, pp. 564-74, 1988 (PubMed).

Davey, Gatter, Ralfkiaer et al.: "Immunophenotyping of non-Hodgkin's lymphomas using a panel of antibodies on paraffin-embedded tissues." in: The American journal of pathology, Vol. 129, Issue 1, pp. 54-63, 1987 (PubMed).

Kester, Toussaint, Punt et al.: "Large induction of type III deiodinase expression after partial hepatectomy in the regenerating mouse and rat liver." in: Endocrinology, Vol. 150, Issue 1, pp. 540-5, 2008 (PubMed).

Otto, Fuchs, Kauselmann et al.: "GPR30 does not mediate estrogenic responses in reproductive organs in mice." in: Biology of reproduction, Vol. 80, Issue 1, pp. 34-41, 2008 (PubMed).

Szabuo, Zhao, Zhou et al.: "The role of Sonic hedgehog of neural origin in thalamic differentiation in the mouse." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 29, Issue 8, pp. 2453-66, 2009 (PubMed).

Katsanou, Milatos, Yiakouvaki et al.: "The RNA-binding protein Elavl1/HuR is essential for placental branching morphogenesis and embryonic development." in: Molecular and cellular biology, Vol. 29, Issue 10, pp. 2762-76, 2009 (PubMed).

Szabuo, Zhao, Cankaya et al.: "Role of neuroepithelial Sonic hedgehog in hypothalamic patterning." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 29, Issue 21, pp. 6989-7002, 2009 (PubMed).

Yeh, Li, Chen et al.: "Antitumor activities and pharmacokinetics of silatecans DB-67 and DB-91." in: Pharmacological research : the official journal of the Italian Pharmacological Society, Vol. 61, Issue 2, pp. 108-15, 2010 (PubMed).

Jung, Bae, Kim et al.: "Comparison of flow cytometry and immunohistochemistry in non-radioisotopic murine lymph node assay using bromodeoxyuridine." in: Toxicology letters, Vol. 192, Issue 2, pp. 229-37, 2010 (PubMed).

Martuin-Ibuaunez, Crespo, Urbuan et al.: "Ikaros-1 couples cell cycle arrest of late striatal precursors with neurogenesis of enkephalinergic neurons." in: The Journal of comparative neurology, Vol. 518, Issue 3, pp. 329-51, 2009 (PubMed).

Gagou, Zuazua-Villar, Meuth: "Enhanced H2AX phosphorylation, DNA replication fork arrest, and cell death in the absence of Chk1." in: Molecular biology of the cell, Vol. 21, Issue 5, pp. 739-52, 2010 (PubMed).