Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) Antikörper

Antigen: Protein tyrosine Phosphatase, Receptor Type, C (PTPRC)

Synonyme: LCA, LY5, B220, CD45, T200, CD45R, GP180, Lca, RT7, L-CA, PTPRC, si:dkeyp-47c5.1

Klonalität: Monoklonal (2B11 and PD7-26)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p))

Zertifikate: ISO 9001:2008, ISO 13485:2003

Anbieter: Dako

Produktnummer: ABIN370561

Produktbeschreibung

Weitere Bezeichnung: CD45, Leucocyte Common Antigen

Immunogen: 2B11: Isolated neoplastic cells from a case of T-cell lymphoma/leukaemia (1). PD7/26: Human peripheral blood lymphocytes maintained in T-cell growth factor (1).

Isotyp: IgG1, kappa chain  (Passende Sekundärantikörper)

Klon: 2B11 and PD7-26

Beschreibung: Synonyms for antigen T200, Ly-5. Summary and explanation CD45 is a transmembrane glycoprotein expressed on most nucleated cells of haematopoietic origin. CD45, encoded by a single gene mapped to chromosome 1, has various isoforms based on differential splicing of exons 4, 5 and 6. On human leucocytes, five different isoforms of CD45, named ABC, AB, BC, B and 0, have been identified. These isoforms are recognized by CD45RA, CD45RB, CD45RC and CD45R0 antibodies. The isoforms range in Mr from 180 000 to 220 000. All the CD45 isoforms share the same intracellular segment, which has been shown to have tyrosine phosphatase activity. Various leucocytes express characteristic CD45 isoforms, thus T cells express CD45 isoforms corresponding to their development and activation, B cells predominantly express the ABC isoform, and monocytes and dendritic cells predominantly express the B and 0 isoforms. Granulocytes principally express only the B and 0 isoforms (4).

Spezifität: Anti-CD45 is a mixture of two monoclonal antibodies, clones 2B11 and PD7/26, directed against different epitopes.

Anwendungen

Applikationshinweise: Paraffin sections: The antibody can be used for labelling paraffin-embedded tissue sections fixed in formalin, B5 (2), or Bouin's (1). Pretreatment of tissues with heat-induced epitope retrieval is recommended. Optimal results are obtained with Dako Target Retrieval Solution, code S1700, Dako Target Retrieval Solution, High pH, code S3308, 10 mmol/L citrate buffer, pH 6.0, or 10 mmol/L Tris buffer, 1 mmol/L EDTA, pH 9.0. Pre-treatment of tissues with proteinase K was found destructive of the epitope. The tissue sections should not dry out during the treatment or during the following immunohistochemical staining procedure. Frozen sections and cell preparations: The antibody can be used for labelling acetone-fixed, frozen sections (1), and cell smears. Dilution: Monoclonal Mouse Anti-Human CD45, Leucocyte Common Antigen, code M0701, may be used at a dilution range of 1:50-1:100 when applied on formalin-fixed, paraffin-embedded sections of human tonsil and using 20 minutes heat-induced epitope retrieval in Dako Target Retrieval solution, code S1700, and 30 minutes incubation at room temperature with the primary antibody. Optimal conditions may vary depending on specimen and preparation method, and should be determined by each individual laboratory. The recommended negative control is Dako Mouse IgG1, code X0931, diluted to the same mouse IgG concentration as the primary antibody. Unless the stability of the diluted antibody and negative control has been established in the actual staining procedure, it is recommended to dilute these reagents immediately before use, or dilute in Dako Antibody Diluent, code S0809. Positive and negative controls should be run simultaneously with patient specimen. Visualization: Dako LSAB/HRP kit, code K0679, and Dako EnVision/HRP kits, codes K4004 and K4006, are recommended. For frozen sections and cell preparations, the Dako APAAP kit, code K0670, is a good alternative if endogenous peroxidase staining is a concern. Follow the procedure enclosed with the selected visualization kit. Automation: The antibody is well-suited for immunohistochemical staining using automated platforms, such as the Dako Autostainer. Product-specific limitations Cells labelled by the antibody predominantly display staining of the cell membrane, but cytoplasmic staining may also occur. Labelling of the surface membrane of mammary ductal cells in specimens of fibrocystic disease and fibroadenoma has been reported for the antibody. This expression, however, is not considered to present a differential diagnostic problem that might lead to misidentification (7).

Buffer: Monoclonal mouse antibody provided in liquid form as cell culture supernatant dialysed against 0.05 mol/L Tris/HCl, pH 7.2, and containing 15 mmol/L NaN 3 .

Lagerung: Store at 2-8 °C. Precautions: 1. For professional users. 2. This product contains sodium azide (NaN 3 ), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used. 4. Wear appropriate Personal Protective Equipment to avoid contact with eyes and skin. 5. Unused solution should be disposed of according to local, State and Federal regulations.

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Publikationen

Publikationen: Poppema, Lai, Visser: "Monoclonal antibody OPD4 is reactive with CD45RO, but differs from UCHL1 by the absence of monocyte reactivity." in: The American journal of pathology, Vol. 139, Issue 4, pp. 725-9, 1991 (PubMed).

Davey, Elghetany, Kurec: "Immunophenotyping of hematologic neoplasms in paraffin-embedded tissue sections." in: American journal of clinical pathology, Vol. 93, Issue 4 Suppl 1, pp. S17-26, 1990 (PubMed).

Norton, Ramsay, Smith et al.: "Monoclonal antibody (UCHL1) that recognises normal and neoplastic T cells in routinely fixed tissues." in: Journal of clinical pathology, Vol. 39, Issue 4, pp. 399-405, 1986 (PubMed).

Smith, Brown, Rowe et al.: "Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1." in: Immunology, Vol. 58, Issue 1, pp. 63-70, 1986 (PubMed).

Kandulski, Wex, Kuester et al.: "Chronic mucosal inflammation of the gastric cardia in gastroesophageal reflux disease is not regulated by FOXP3-expressing T cells." in: Digestive diseases and sciences, Vol. 54, Issue 9, pp. 1940-6, 2009 (PubMed).

Said, Nubile, Alomar et al.: "Histologic features of transplanted amniotic membrane: implications for corneal wound healing." in: Ophthalmology, Vol. 116, Issue 7, pp. 1287-95, 2009 (PubMed).

Krishnamurthy, Hassan, Frater et al.: "Pathologic and Clinical Features of Hodgkin Lymphoma-Like Posttransplant Lymphoproliferative Disease." in: International journal of surgical pathology, 2009 (PubMed).

Tigerstedt, Savolainen-Peltonen, Lehti et al.: "Vascular cell kinetics in response to intimal injury ex vivo." in: Journal of vascular research, Vol. 47, Issue 1, pp. 35-44, 2009 (PubMed).

Higgins, Zehnder, Chen et al.: "The histological development of acute antibody-mediated rejection in HLA antibody-incompatible renal transplantation." in: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, Vol. 25, Issue 4, pp. 1306-12, 2010 (PubMed).

Kitai, Matsuda, Adachi et al.: "Epstein-Barr virus-associated primary central nervous system lymphoma in the Japanese population." in: Neurologia medico-chirurgica, Vol. 50, Issue 2, pp. 114-8, 2010 (PubMed).