Insulin (INS) Antikörper

Antigen: Insulin (INS)

Synonyme: ILPR, IRDN, INS, MGC159719, ins1, xins, ins1-a, Insulin

Klonalität: Polyklonal

Wirt: Meerschweinchen

Applikation: Immunhistochemie (IHC)

Zertifikate: ISO 9001:2008, ISO 13485:2003

Anbieter: Dako

Produktnummer: ABIN370537

Produktbeschreibung

Weitere Bezeichnung: Insulin

Immunogen: Porcine pancreatic insulin

Beschreibung: Insulin is one of seven known polypeptide hormones produced in the pancreas. Insulin, secreted by B-cells of the islets of Langerhans, participates in glucose utilization, protein synthesis and in the formation and storage of neutral lipids. 1

Spezifität: The antibody cross-reacts with insulin from several mammalian species. as determined by radioimmunoassay was 100% for human insulin, 100% for porcine insulin and less than 0.05% for glucagon and human growth hormone. This product has been optimized for use on human tissues. For more information on insulin detection and pancreatic tumors, please see references 3–8.

Anwendungen

Applikationshinweise: Polyclonal guinea pig anti-insulin is intended for laboratory use to identify qualitatively by light microscopy insulin positive cells in normal and neoplastic tissues using immunohistochemical (IHC) test methods. The clinical interpretation of any positive staining or its absence should be complemented by morphological and histological studies with proper controls. Evaluations should be made within the context of the patient's clinical history and other diagnostic tests by a qualified individual. Paraffin Sections Guinea pig anti-insulin can be used on formalin-fixed, paraffin-embedded tissue sections. Pretreatment of tissue with proteolytic enzymes is not required. Follow the recommended procedure for the detection system selected. Staining interpretation The cellular staining pattern for anti-insulin is cytoplasmic. (105462-003) 303255EFG_001 p. 2/4 Normal Tissues This antibody labels the cytoplasm of insulin-producing B-cells. Abnormal Tissues Insulin producing islet cell tumors, hyperplastic islet cells and islet cells originating in pancreatic ductules are positively labelled. 2 Mesenchymal tumors are not reactive.

Buffer: Guinea pig anti-insulin is an unfractionated guinea pig antiserum in 0.05 mol/L Tris-HCl, pH 7.6, and 0.015 mol/L sodium azide.

Lagerung: Store at 2-8 °C. Precautions: 1. For professional users. 2. This product contains sodium azide (NaN 3 ), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, NaN 3 may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used. 4. Wear appropriate Personal Protective Equipment to avoid contact with eyes and skin. 5. Unused reagents should be disposed of according to local, State, and Federal regulations.

Forschungsgebiet: Diabetes, Hormone

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Publikationen

Publikationen: Creutzfeldt, Arnold, Creutzfeldt et al.: "Pathomorphologic, biochemical, and diagnostic aspects of gastrinomas (Zollinger-Ellison syndrome)." in: Human pathology, Vol. 6, Issue 1, pp. 47-76, 1975 (PubMed).

Parsons, Erlandsen, Hegre et al.: "Central and peripheral localization of somatostatin. Immunoenzyme immunocytochemical studies." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 24, Issue 7, pp. 872-82, 1976 (PubMed).

Erlandsen, Hegre, Parsons et al.: "Pancreatic islet cell hormones distribution of cell types in the islet and evidence for the presence of somatostatin and gastrin within the D cell." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 24, Issue 7, pp. 883-97, 1976 (PubMed).

Alumets, Sundler, Huakanson: "Distribution, ontogeny and ultrastructure of somatostatin immunoreactive cells in the pancreas and gut." in: Cell and tissue research, Vol. 185, Issue 4, pp. 465-79, 1978 (PubMed).

Dubois: "Immunoreactive somatostatin is present in discrete cells of the endocrine pancreas." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 72, Issue 4, pp. 1340-3, 1975 (PubMed).