Maus anti-Human IgE (Fc Region) Antikörper

Produktnummer ABIN363706

Produktdetails

Target: IgE

Bindungsspezifität: Fc Region

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Applikation: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func)

Spezifität: Human IgE, Fce3-4, no cross-ractivity with other human or murine Ab isotypes

Produktmerkmale: IGHE, chromosome 14q32.33

Aufreinigung: Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B

Reinheit: > 95 %, tested by analytical Gel Permeation Chromatography

Sterilität: Sterile filtered

Immunogen: purified human myeloma IgE

Klon: BSW17-NBS01

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: For functional cellular assays (mast cell / basophil activation), when used at dilutions < 1:100, the sodium azide free formulation is recommended. Quality assurance: Reactivity > 99 %, compared to calibrator (Internal Standard mAb BSW17), tested by ELISA binding to immobilized human Standard IgE (SUS-11)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS, pH=7.2, sterile filtered, contains no NaN3

Konservierungsmittel: Azide free

Handhabung: Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.

Lagerung: 4 °C

Referenzen

Rudolf, Zuercher, Nechansky, Ruf, Vogel, Miescher, Stadler, Kricek: "Molecular basis for nonanaphylactogenicity of a monoclonal anti-IgE antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 2, pp. 813-9, (2000) (PubMed).

Antigendetails

Target: IgE

Abstract: IgE Produkte

Hintergrund: The antibody recognizes a conformational epitope within the Ce3 domain of human IgE spanning towards the amino-terminal part of the Ce4 domain. It blocks the binding of IgE to its high affinity receptor on mast cells and basophils. It is therefore suited as an inhibitory reference compound for mast cell modulator profiling and basic research on IgE mediated mast cell receptor signaling.