The antibody detects endogenous level of total P38MAPK protein.
Aufreinigung
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen. Specificity/Sensitivity P38MAPK (Ab-182) antibody detects endogenous levels of total P38MAPK protein.
Immunogen
Peptide sequence around AA 180-184 (T-G-Y-V-A) derived from Human P38 MAPK. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
MAPK14
Reaktivität: Human
WB, IHC, IF, IP
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
Western blotting: 1:500-1:1000
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Su, Gu, Wang, Wang: "Lidocaine attenuates proinflammatory cytokine production induced by extracellular adenosine triphosphate in cultured rat microglia." in: Anesthesia and analgesia, Vol. 111, Issue 3, pp. 768-74, (2010) (PubMed).
Gu, Su, Tian, Wang: "Attenuating phosphorylation of p38 MAPK in the activated microglia: a new mechanism for intrathecal lidocaine reversing tactile allodynia following chronic constriction injury in rats." in: Neuroscience letters, Vol. 431, Issue 2, pp. 129-34, (2008) (PubMed).
Target
MAPK14
(Mitogen-Activated Protein Kinase 14 (MAPK14))
Responds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.