The antibody detects endogenous level of TrkB only when phosphorylated at tyrosine 705.
Aufreinigung
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Immunogen
Peptide sequence around phosphorylation site of pTyr705 (T-D-Y P-Y-R) derived from Human TrkB. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
NTRK2
Reaktivität: Human
WB, PLA
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
Western blotting: 1:500-1:1000
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Hill, Wu, Gogos, van den Buuse: "Sex-dependent alterations in BDNF-TrkB signaling in the hippocampus of reelin heterozygous mice: a role for sex steroid hormones." in: Journal of neurochemistry, Vol. 126, Issue 3, pp. 389-99, (2013) (PubMed).
Hill, van den Buuse: "Sex-dependent and region-specific changes in TrkB signaling in BDNF heterozygous mice." in: Brain research, Vol. 1384, pp. 51-60, (2011) (PubMed).
Target
TRKB (NTRK2)
(Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2))
Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1.