TRKB Antikörper (pTyr705)

Produktnummer ABIN362425

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch TRKB in WB. Es zeigt Reaktivität gegenüber Proben von Human, Ratte und Maus. Es wurde in 2+ Publikationen zitiert.

Kurzübersicht für TRKB Antikörper (pTyr705) (ABIN362425)

Target: TRKB (NTRK2) (Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TRKB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-TRKB Antikörper

Bindungsspezifität: pTyr705

Spezifität: The antibody detects endogenous level of TrkB only when phosphorylated at tyrosine 705.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pTyr705 (T-D-Y P-Y-R) derived from Human TrkB. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 1:500-1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Referenzen für anti-TRKB Antikörper (ABIN362425)

Hill, Wu, Gogos, van den Buuse: "Sex-dependent alterations in BDNF-TrkB signaling in the hippocampus of reelin heterozygous mice: a role for sex steroid hormones." in: Journal of neurochemistry, Vol. 126, Issue 3, pp. 389-99, (2013) (PubMed).

Hill, van den Buuse: "Sex-dependent and region-specific changes in TrkB signaling in BDNF heterozygous mice." in: Brain research, Vol. 1384, pp. 51-60, (2011) (PubMed).

Details zu TRKB

Target: TRKB (NTRK2) (Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2))

Andere Bezeichnung: TrkB

Hintergrund: Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1.

Molekulargewicht: 140 kDa

UniProt: Q16620

Pathways: RTK Signalweg, Neurotrophin Signalübertragung, cAMP Metabolic Process, Skeletal Muscle Fiber Development, Feeding Behaviour, Dicarboxylic Acid Transport