Phosphotyrosine (0) Antikörper

Antigen: Phosphotyrosine (0)

Klonalität: Monoklonal (13F9)

Wirt: Armenischer Hamster

Reaktivität: Alle Spezies

Applikation: ELISA, Durchflusszytometrie (FACS), Immunhistochemie (IHC), Immunpräzipitation (IP)

Produktnummer: ABIN361806

Produktbeschreibung

Weitere Bezeichnung: Phospho-tyrosine

Immunogen: Phosphotyrosine conjugated to KLH

Isotyp: IgG1 kappa  (Passende Sekundärantikörper)

Klon: 13F9

Beschreibung: Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein

Spezifität: Reacts with phosphotyrosine, and detects the presence of phosphotyrosine in proteins of both unstimulated and stimulated cell lysates. Does not cross react with phosphoserine or phosphothreonine. Species cross-reactivity: Not species specific.

Anwendungen

Applikationshinweise: Working dilution 1:2000 to 1:10000

Buffer: Clarified ascites containing 0.02%NaN3

Lagerung: -20°C, 1 year+, shipped on cold packs or ambient

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Ross, Baltimore, Eisen: "Phosphotyrosine-containing proteins isolated by affinity chromatography with antibodies to a synthetic hapten." in: Nature, Vol. 294, Issue 5842, pp. 654-6, 1982 (PubMed).

Frackelton, Ross, Eisen: "Characterization and use of monoclonal antibodies for isolation of phosphotyrosyl proteins from retrovirus-transformed cells and growth factor-stimulated cells." in: Molecular and cellular biology, Vol. 3, Issue 8, pp. 1343-52, 1983 (PubMed).

Ostrovsky, Maloy: "Protein phosphorylation on serine, threonine, and tyrosine residues modulates membrane-protein interactions and transcriptional regulation in Salmonella typhimurium." in: Genes & development, Vol. 9, Issue 16, pp. 2034-41, 1995 (PubMed).

Pawson, Saxton: "Signaling networks--do all roads lead to the same genes?" in: Cell, Vol. 97, Issue 6, pp. 675-8, 1999 (PubMed).

Blume-Jensen, Hunter: "Oncogenic kinase signalling." in: Nature, Vol. 411, Issue 6835, pp. 355-65, 2001 (PubMed).

Downward: "The ins and outs of signalling." in: Nature, Vol. 411, Issue 6839, pp. 759-62, 2001 (PubMed).

Goto, Kiyono, Tomono et al.: "Complex formation of Plk1 and INCENP required for metaphase-anaphase transition." in: Nature cell biology, Vol. 8, Issue 2, pp. 180-7, 2006 (PubMed).