Phosphotyrosine (0) Antikörper

Antigen: Phosphotyrosine (0)

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Diverse Spezies

Applikation: Western Blot (WB), ELISA, Immunpräzipitation (IP), Immunhistochemie (IHC)

Produktnummer: ABIN361758

Produktbeschreibung

Weitere Bezeichnung: Phospho-tyrosine

Immunogen: Phosphotyrosine conjugated to KLH

Format: Antiserum

Beschreibung: Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein

Spezifität: Recognizes proteins phosphorylated on tyrosine residues. Does not cross-react with phosphoserine or threonine. Species cross-reactivity: Multi-species.

Anwendungen

Applikationshinweise: WB 2 ug/mL, ELISA 0.5 ug/ml, IP 10 ug/mg sample

Konzentration: 250

Buffer: Rabbit antiserum in TBS, 0.1%NaN3

Lagerung: -20°C, 1 year+, shipped on cold packs or ambient

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Ross, Baltimore, Eisen: "Phosphotyrosine-containing proteins isolated by affinity chromatography with antibodies to a synthetic hapten." in: Nature, Vol. 294, Issue 5842, pp. 654-6, 1982 (PubMed).

Frackelton, Ross, Eisen: "Characterization and use of monoclonal antibodies for isolation of phosphotyrosyl proteins from retrovirus-transformed cells and growth factor-stimulated cells." in: Molecular and cellular biology, Vol. 3, Issue 8, pp. 1343-52, 1983 (PubMed).

Ostrovsky, Maloy: "Protein phosphorylation on serine, threonine, and tyrosine residues modulates membrane-protein interactions and transcriptional regulation in Salmonella typhimurium." in: Genes & development, Vol. 9, Issue 16, pp. 2034-41, 1995 (PubMed).

Pawson, Saxton: "Signaling networks--do all roads lead to the same genes?" in: Cell, Vol. 97, Issue 6, pp. 675-8, 1999 (PubMed).

Blume-Jensen, Hunter: "Oncogenic kinase signalling." in: Nature, Vol. 411, Issue 6835, pp. 355-65, 2001 (PubMed).

Downward: "The ins and outs of signalling." in: Nature, Vol. 411, Issue 6839, pp. 759-62, 2001 (PubMed).

Goto, Kiyono, Tomono et al.: "Complex formation of Plk1 and INCENP required for metaphase-anaphase transition." in: Nature cell biology, Vol. 8, Issue 2, pp. 180-7, 2006 (PubMed).