BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11) Antikörper

Antigen: BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11)

Synonyme: BAM, BIM, BOD, BimL, BimEL, BIM-beta6, BIM-beta7, BIM-alpha6, Bim, Bod, 1500006F24Rik, bim

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: Western Blot (WB), Immunhistochemie (IHC)

Produktnummer: ABIN361749

Produktbeschreibung

Weitere Bezeichnung: Bim/Bod

Immunogen: Internal central amino acids of human Bim

Format: Affinity-purified

Beschreibung: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bim/BOD is a group of three splice variants, BimEL, BimL and BimS, with apparent molecular masses of ~23, 16, and 13 kDa, respectively. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bim or BOD in human, mouse and rat (1,2). Bim/BOD interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. Bim/BOD induces apoptosis. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines (1,2).

Spezifität: Detects a 23 kD protein corresponding to the molecular mass of Bim on SDS PAGE immunoblots. Species cross-reactivity: Human, mouse, rat.

Anwendungen

Applikationshinweise: Suggested dilution 1: 1000 for Western blots, 1:50 for IHC.

Konzentration: 1mg/mL

Buffer: Affinity purified in PBS with 0.02% Sodium Azide

Lagerung: -20°C, 1 year+, shipped on cold packs or ambient

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Bim-Bod (IN), Human Skin Cancer Cells

Bim-BOD (IN), Bim in K562 (K) and A549 (A) whole cell lysates

Publikationen

Publikationen: OConnor, Strasser, OReilly et al.: "Bim: a novel member of the Bcl-2 family that promotes apoptosis." in: The EMBO journal, Vol. 17, Issue 2, pp. 384-95, 1998 (PubMed).

Hsu, Lin, Hsueh: "BOD (Bcl-2-related ovarian death gene) is an ovarian BH3 domain-containing proapoptotic Bcl-2 protein capable of dimerization with diverse antiapoptotic Bcl-2 members." in: Molecular endocrinology (Baltimore, Md.), Vol. 12, Issue 9, pp. 1432-40, 1998 (PubMed).