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HSP90 Antikörper

HSP90 Reaktivität: Human IP, IF, ICC Wirt: Maus Monoclonal 8D3 unconjugated
Produktnummer ABIN361731
  • Target Alle HSP90 Antikörper anzeigen
    HSP90 (Heat Shock Protein 90 (HSP90))
    Reaktivität
    • 121
    • 63
    • 63
    • 33
    • 26
    • 21
    • 20
    • 20
    • 12
    • 10
    • 10
    • 10
    • 10
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Wirt
    • 97
    • 66
    • 3
    • 1
    Maus
    Klonalität
    • 100
    • 67
    Monoklonal
    Konjugat
    • 58
    • 12
    • 11
    • 11
    • 11
    • 11
    • 10
    • 10
    • 9
    • 9
    • 7
    • 4
    • 4
    Dieser HSP90 Antikörper ist unkonjugiert
    Applikation
    • 139
    • 104
    • 93
    • 70
    • 61
    • 57
    • 33
    • 20
    • 13
    • 10
    • 2
    • 2
    • 1
    Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)
    Spezifität
    Detects 90 kDa. Co-immunoprecipitates HSP90 complexes, including HSP70, Hop, Ah receptors, glucocorticoid receptors, heme-regulated eukaryotic initiation factor 2α (eIF-2α) kinase (HRI).
    Kreuzreaktivität
    Human, Maus, Kaninchen, Ratte
    Aufreinigung
    PEG Purified
    Immunogen
    Ah receptor (Aryl hydrocarbon receptor)
    Klon
    8D3
    Isotyp
    IgM
    Top Product
    Discover our top product HSP90 Primärantikörper
  • Applikationshinweise
    • ICC/IF (1:100)
    • IP (1:1000)
    • optimal dilutions for assays should be determined by the user.
    Kommentare

    Goat anti-mouse IgM was used to bind 25 μl of protein G-Sepharose. ABIN361731 IgM from 0.5 ml of high speed supernatant medium was loaded onto the IgG resin and incubated with 100 μl of rabbit reticulocyte lysate for 30 min. at 30C. After washing (4X1 ml), bound proteins were resolved on SDS PAGE, including HSP90, HSP70 and Hop.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1 mg/mL
    Buffer
    PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    -20°C
  • Shimamura, Perera, Foley, Sang, Rodig, Inoue, Chen, Li, Carretero, Li, Sinha, Carey, Borgman, Jimenez, Meyerson, Ying, Barsoum, Wong, Shapiro: "Ganetespib (STA-9090), a nongeldanamycin HSP90 inhibitor, has potent antitumor activity in in vitro and in vivo models of non-small cell lung cancer." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 18, Issue 18, pp. 4973-85, (2012) (PubMed).

    Ardi, Alexander, Johnson, McAlpine: "Macrocycles that inhibit the binding between heat shock protein 90 and TPR-containing proteins." in: ACS chemical biology, Vol. 6, Issue 12, pp. 1357-66, (2011) (PubMed).

  • Target
    HSP90 (Heat Shock Protein 90 (HSP90))
    Andere Bezeichnung
    HSP90 (HSP90 Produkte)
    Synonyme
    EL52 antikoerper, HSP86 antikoerper, HSP89A antikoerper, HSP90A antikoerper, HSP90N antikoerper, HSPC1 antikoerper, HSPCA antikoerper, HSPCAL1 antikoerper, HSPCAL4 antikoerper, HSPN antikoerper, Hsp89 antikoerper, Hsp90 antikoerper, LAP2 antikoerper, git10 antikoerper, swo1 antikoerper, HSP90 antikoerper, htpG antikoerper, SCBAC25F8.08 antikoerper, 23.m06066 antikoerper, 17.m07646 antikoerper, HSP90-1 antikoerper, 143198_at antikoerper, 83 antikoerper, 83K HSP antikoerper, DMHSP82 antikoerper, E(sev)3A antikoerper, E(sina)2 antikoerper, HSP82 antikoerper, HSP83 antikoerper, ORF1 antikoerper, Su(Raf)3A antikoerper, anon-EST:Liang-2.53 antikoerper, anon-WO0068693 antikoerper, anon-WO0140519.209 antikoerper, clone 2.53 antikoerper, en(lz)3C/4C antikoerper, hsp84 antikoerper, l(3)j5C2 antikoerper, ms(3)08445 antikoerper, stc antikoerper, DmelCG1242 antikoerper, CG1242 antikoerper, Hsp86 antikoerper, Hspca antikoerper, 86kDa antikoerper, 89kDa antikoerper, AL024080 antikoerper, AL024147 antikoerper, Hsp86-1 antikoerper, hsp4 antikoerper, hsp86 antikoerper, hsp89 antikoerper, hsp90 antikoerper, hsp90a antikoerper, hspc1 antikoerper, hspca antikoerper, hspn antikoerper, lap2 antikoerper, Hsp90alpha antikoerper, heat shock protein 90 alpha family class A member 1 antikoerper, heat shock protein Hsp90 antikoerper, Hsp90 chaperone antikoerper, Heat shock protein 90 antikoerper, heat shock protein 90 antikoerper, chaperone protein HtpG antikoerper, molecular chaperone HtpG antikoerper, Heat Shock Protein 90 antikoerper, uncharacterized LOC100384473 antikoerper, Heat shock protein 83 antikoerper, heat shock protein 90, alpha (cytosolic), class A member 1 antikoerper, heat shock protein 90 alpha family class B member 1 antikoerper, heat shock protein 90kDa alpha family class A member 1 L homeolog antikoerper, heat shock protein HSP 90-alpha antikoerper, HSP90AA1 antikoerper, hsp90 antikoerper, HSP90 antikoerper, daf-21 antikoerper, htpG antikoerper, SCO7516 antikoerper, MAP_RS10510 antikoerper, TP04_0646 antikoerper, TP01_0934 antikoerper, APH_RS03525 antikoerper, GbCGDNIH1_0315 antikoerper, MAV_2118 antikoerper, HSP90C antikoerper, BBOV_IV008400 antikoerper, BBOV_III007380 antikoerper, ACICU_00312 antikoerper, ECL_01244 antikoerper, YE105_C1172 antikoerper, pco153543(105) antikoerper, Hsp83 antikoerper, Hsp90aa1 antikoerper, HSP90AB1 antikoerper, hsp90aa1.1.L antikoerper, LOC108698781 antikoerper
    Hintergrund
    HSP90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. From a functional perspective, HSP90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex (1-4). Despite its label of being a heat-shock protein, HSP90 is one of the most highly expressed proteins in unstressed cells (1-2 % of cytosolic protein). It carries out a number of housekeeping functions - including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the HSP90-regulated proteins that have been discovered to date are involved in cell signaling (5-6). The number of proteins now know to interact with HSP90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase (5). When bound to ATP, HSP90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. In most cases, HSP90-interacting proteins have been shown to co-precipitate with HSP90 when carrying out immunoadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in HSP90 expression or HSP90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit HSP90 function (7). For more information visit our HSP90 Scientific Resource Guide at http://www.HSP90.ca.
    Gen-ID
    3326
    NCBI Accession
    NP_031381
    UniProt
    P08238
    Pathways
    M Phase, Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
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