HSF2 Antikörper

Produktnummer ABIN361728

Produktdetails anti-HSF2 Antikörper

Target: HSF2 (Heat shock factor protein 2 (HSF2))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser HSF2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Antibody Array (AA)

Spezifität: Detects ~69 kDa.

Kreuzreaktivität: Rind (Kuh), Hund, Meerschweinchen, Hamster, Human, Affe, Maus, Schwein, Kaninchen, Ratte, Schaf

Aufreinigung: Protein G Purified

Immunogen: Purified recombinant mouse HSF2 protein

Klon: 3E2

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

• WB (1:250)
• ICC/IF (1:200)
• optimal dilutions for assays should be determined by the user.

Kommentare:

4 μg/ml of ABIN361727 was sufficient for detection of HSF2 in 20 μg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Rabbit anti-rat IgG: AP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Details zu HSF2

Target: HSF2 (Heat shock factor protein 2 (HSF2))

Andere Bezeichnung: HSF2 (HSF2 Produkte)

Synonyme: AI661205 antikoerper, HSF 2 antikoerper, HSTF 2 antikoerper, HSF3B antikoerper, HSTF3B antikoerper, heat shock factor 2 antikoerper, heat shock transcription factor 2 antikoerper, Hsf2 antikoerper, HSF2 antikoerper

Hintergrund: HSF2, or heat shock factor 2, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the N-terminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6). There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transciptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11).

Gen-ID: 15500

NCBI Accession: NP_001129036