Ribosomal Protein S6 Kinase, Polypeptide 1 (RPS6KB1) Antikörper

Antigen: Ribosomal Protein S6 Kinase, Polypeptide 1 (RPS6KB1)

Synonyme: 7084, DS6K, Dp70S6k, Dp70[s6k], S6K, dps6k, fs(3)07084, l(3)07084, p70 S6K, p70/S6K, p70S6K, p70[S6 kinase], p70[S6k], p70[S6kinase], s6k11, DmelCG10539, CG10539, S6K1, 70kDa, p70s6k, AA959758, AI256796, AI314060, p70/85s6k, 2610318I15Rik, 4732464A07Rik, p70s6k-A, pkacb, MGC80071, XPKAbeta

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Maus, Meerschweinchen, Rind (Kuh)

Applikation: Western Blot (WB), Immunpräzipitation (IP), ELISA

Produktnummer: ABIN361700

Produktbeschreibung

Produktmerkmale: Detects a ~70kDa protein corresponding to the molecular mass of p70 S6K on SDS PAGE immunoblots.

Weitere Bezeichnung: p70 S6K

Gen-ID: NP_003943.2, 6199

Immunogen: Human p70 S6K c-terminal peptide –KLH conjugates

Format: Liquid

Beschreibung: Ribosomal S6 (S6K) belongs to the AGC family of Serine/Threonine protein kinases. There are two forms of S6K, alpha and beta, which have cytoplasmic and nuclear variants derived from alternative splicing (1). The activity of S6K is regulated by phosphorylation/ dephosphorylation events in cellular responses to various extracellular stimuli. Its activity has also been shown to be dependent on nutrient availability (3-6). The mechanism of activation of S6K is a multi-step process which is achieved by phosphorylating the 40S ribosomal protein S6, which then upregulates the translation of mRNA transcripts containing an oligopryimidine tract at the 5’ transcriptional start site (3-6). Recently, a highly homologous ribosomal S6 kinase, termed S6K2, was identified. It has 70% amino acid identity in the overall sequence with S6K1, and highly conserved potential phosphorylation sites of S6K1. However, the N- and C-terminal domains of S6K2 are quite different (2). As a result, S6K is a key player in the regulation of cell size, growth and glucose homeostasis.
Synonyms: p70 S6 kinase beta (phospho T229), p70-beta, p70S6Kb, S6K beta, S6K2, SRK, STK14B

Spezifität: Detects a ~70kDa protein, corresponding to the molecular mass of p70 S6K on SDS-PAGE immunoblots. Species cross-reactivity: Human, Mouse, Rat, Bovine.

Synonyme: 7084, DS6K, Dp70S6k, Dp70[s6k], S6K, dps6k, fs(3)07084, l(3)07084, p70 S6K, p70/S6K, p70S6K, p70[S6 kinase], p70[S6k], p70[S6kinase], s6k11, DmelCG10539, CG10539, S6K1, 70kDa, p70s6k, AA959758, AI256796, AI314060, p70/85s6k, 2610318I15Rik, 4732464A07Rik, p70s6k-A, pkacb, MGC80071, XPKAbeta

Anwendungen

Applikationshinweise: 1ug/mL was needed for western blot analysis

Konzentration: 250µg/mL

Buffer: TBS, 50% glycerol, 0.09% sodium azide

Lagerung: -20 °C

Storage Shipping Temp Max: Blue Ice or 4 °C

Forschungsgebiet: Zelluläre Signaltransduktion

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

p70 S6K, mouse brain (R), neg CT (L)

Publikationen

Publikationen: Kawasome, Papst, Webb et al.: "Targeted disruption of p70(s6k) defines its role in protein synthesis and rapamycin sensitivity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 9, pp. 5033-8, 1998 (PubMed).

Gout, Minami, Hara et al.: "Molecular cloning and characterization of a novel p70 S6 kinase, p70 S6 kinase beta containing a proline-rich region." in: The Journal of biological chemistry, Vol. 273, Issue 46, pp. 30061-4, 1998 (PubMed).

Shima, Pende, Chen et al.: "Disruption of the p70(s6k)/p85(s6k) gene reveals a small mouse phenotype and a new functional S6 kinase." in: The EMBO journal, Vol. 17, Issue 22, pp. 6649-59, 1999 (PubMed).

Boyer, Quintanilla, Lee-Fruman: "Regulation of catalytic activity of S6 kinase 2 during cell cycle." in: Molecular and cellular biochemistry, Vol. 307, Issue 1-2, pp. 59-64, 2007 (PubMed).