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Lysine (lys) (methylated) Antikörper (Biotin)

lys WB, ICC, IF, IP, ELISA Wirt: Kaninchen Polyclonal Biotin
Produktnummer ABIN361688
  • Target
    Lysine (lys)
    Bindungsspezifität
    • 23
    • 10
    methylated
    Reaktivität
    Bitte anfragen
    Wirt
    • 21
    • 12
    Kaninchen
    Klonalität
    • 21
    • 12
    Polyklonal
    Konjugat
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    Biotin
    Applikation
    • 33
    • 32
    • 32
    • 32
    • 32
    • 11
    • 1
    Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA
    Spezifität
    Detects proteins containing methylated lysine residues.
    Aufreinigung
    Protein A Purified
    Immunogen
    Methylated KLH Conjugated
  • Applikationshinweise
    • WB (1:5000)
    • ICC/IF (1:50)
    • optimal dilutions for assays should be determined by the user.
    Kommentare

    0.2-0.5 μg/ml of SPC-159 was sufficient for detection of the methylated histone by western blot analysis using melanoma cells in TBSt.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.25 mg/mL
    Buffer
    PBS, 50 % glycerol, Storage buffer may change when conjugated
    Lagerung
    4 °C
    Informationen zur Lagerung
    Conjugated antibodies should be stored at 4°C
  • Target
    Lysine (lys)
    Andere Bezeichnung
    Lysine
    Synonyme
    lysine antikoerper, lys antikoerper
    Substanzklasse
    Amino Acid
    Hintergrund
    Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nε-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3). The regulation of protein acetylation status is impaired in the pathologies of cancer and polyglutamine diseases (4), and HDACs have become promising targets for anti-cancer drugs currently in development (5).
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