HSP90 Antikörper

Produktnummer ABIN361665

Produktdetails anti-HSP90 Antikörper

Target: HSP90 (Heat Shock Protein 90 (HSP90))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HSP90 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Spezifität: Detects ~90 kDa. This antibody detects both α and β forms of HSP90 equally well.

Kreuzreaktivität: Human, Maus, Pflanzen, Ratte

Aufreinigung: Protein G Purified

Immunogen: Recombinant Human HSP90 purified from E.coli

Klon: 4F3-E8

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise:

• WB (1:2000)
• IHC (1:100)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Kommentare:

0.5 μg/ml of ABIN361665 was sufficient for detection of HSP90alpha in 20 μg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Referenzen für anti-HSP90 Antikörper (ABIN361665)

Falchi, Varricchio, Martelli, Marra, Picconi, Tafuri, Girelli, Uversky, Migliaccio: "The Calreticulin control of human stress erythropoiesis is impaired by JAK2V617F in polycythemia vera." in: Experimental hematology, Vol. 50, pp. 53-76, (2017) (PubMed).

Fellerer, Schweiger, Schöngruber, Soll, Schwenkert: "Cytosolic HSP90 cochaperones HOP and FKBP interact with freshly synthesized chloroplast preproteins of Arabidopsis." in: Molecular plant, Vol. 4, Issue 6, pp. 1133-45, (2011) (PubMed).

Details zu HSP90

Target: HSP90 (Heat Shock Protein 90 (HSP90))

Andere Bezeichnung: HSP90 (HSP90 Produkte)

Synonyme: EL52 antikoerper, HSP86 antikoerper, HSP89A antikoerper, HSP90A antikoerper, HSP90N antikoerper, HSPC1 antikoerper, HSPCA antikoerper, HSPCAL1 antikoerper, HSPCAL4 antikoerper, HSPN antikoerper, Hsp89 antikoerper, Hsp90 antikoerper, LAP2 antikoerper, git10 antikoerper, swo1 antikoerper, HSP90 antikoerper, htpG antikoerper, SCBAC25F8.08 antikoerper, 23.m06066 antikoerper, 17.m07646 antikoerper, HSP90-1 antikoerper, 143198_at antikoerper, 83 antikoerper, 83K HSP antikoerper, DMHSP82 antikoerper, E(sev)3A antikoerper, E(sina)2 antikoerper, HSP82 antikoerper, HSP83 antikoerper, ORF1 antikoerper, Su(Raf)3A antikoerper, anon-EST:Liang-2.53 antikoerper, anon-WO0068693 antikoerper, anon-WO0140519.209 antikoerper, clone 2.53 antikoerper, en(lz)3C/4C antikoerper, hsp84 antikoerper, l(3)j5C2 antikoerper, ms(3)08445 antikoerper, stc antikoerper, DmelCG1242 antikoerper, CG1242 antikoerper, Hsp86 antikoerper, Hspca antikoerper, 86kDa antikoerper, 89kDa antikoerper, AL024080 antikoerper, AL024147 antikoerper, Hsp86-1 antikoerper, hsp4 antikoerper, hsp86 antikoerper, hsp89 antikoerper, hsp90 antikoerper, hsp90a antikoerper, hspc1 antikoerper, hspca antikoerper, hspn antikoerper, lap2 antikoerper, Hsp90alpha antikoerper, heat shock protein 90 alpha family class A member 1 antikoerper, heat shock protein Hsp90 antikoerper, Hsp90 chaperone antikoerper, Heat shock protein 90 antikoerper, heat shock protein 90 antikoerper, chaperone protein HtpG antikoerper, molecular chaperone HtpG antikoerper, Heat Shock Protein 90 antikoerper, uncharacterized LOC100384473 antikoerper, Heat shock protein 83 antikoerper, heat shock protein 90, alpha (cytosolic), class A member 1 antikoerper, heat shock protein 90 alpha family class B member 1 antikoerper, heat shock protein 90kDa alpha family class A member 1 L homeolog antikoerper, heat shock protein HSP 90-alpha antikoerper, HSP90AA1 antikoerper, hsp90 antikoerper, HSP90 antikoerper, daf-21 antikoerper, htpG antikoerper, SCO7516 antikoerper, MAP_RS10510 antikoerper, TP04_0646 antikoerper, TP01_0934 antikoerper, APH_RS03525 antikoerper, GbCGDNIH1_0315 antikoerper, MAV_2118 antikoerper, HSP90C antikoerper, BBOV_IV008400 antikoerper, BBOV_III007380 antikoerper, ACICU_00312 antikoerper, ECL_01244 antikoerper, YE105_C1172 antikoerper, pco153543(105) antikoerper, Hsp83 antikoerper, Hsp90aa1 antikoerper, HSP90AB1 antikoerper, hsp90aa1.1.L antikoerper, LOC108698781 antikoerper

Hintergrund: HSP90 is an abundantly and ubiquitously expressed heat shock protein. It is understood to exist in two principal forms α and β, which share 85 % sequence amino acid homology. The two isoforms of HSP90 are expressed in the cytosolic compartment (1). Despite the similarities, HSP90α exists predominantly as a homodimer while HSP90β exists mainly as a monomer (2). From a functional perspective, HSP90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex (3-6). Furthermore, HSP90 is highly conserved between species, having 60 % and 78 % amino acid similarity between mammalian and the corresponding yeast and Drosophila proteins, respectively. HSP90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. Despite its label of being a heat-shock protein, HSP90 is one of the most highly expressed proteins in unstressed cells (1-2 % of cytosolic protein). It carries out a number of housekeeping functions - including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the HSP90-regulated proteins that have been discovered to date are involved in cell signaling (7-8). The number of proteins now know to interact with HSP90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase (5). When bound to ATP, HSP90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. In most cases, HSP90-interacting proteins have been shown to co-precipitate with HSP90 when carrying out immunoadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in HSP90 expression or HSP90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit HSP90 function (9). For more information visit our HSP90 Scientific Resource Guide at http://www.HSP90.ca.

Gen-ID: 3320

NCBI Accession: NP_001017963

UniProt: P07900

Pathways: M Phase, Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals