Hsp40, YDJ1 Antikörper

Antigen: Hsp40, YDJ1

Klonalität: Monoklonal (2A7-H6)

Wirt: Maus

Reaktivität: Yeast (Pichia pastoris)

Applikation: Western Blot (WB), Immunpräzipitation (IP), ELISA

Produktnummer: ABIN361662

Produktbeschreibung

Produktmerkmale: Detects ~40kDa.

Gen-ID: 855661

Swiss-Prot: P25491

Immunogen: Full length protein Hsp40 (YDJ1)

Kreuzreaktivität (Details): Does not cross react with human, mouse or rat,

Format: Liquid

Isotyp: IgG1 kappa  (Passende Sekundärantikörper)

Klon: 2A7-H6

Beschreibung: Human Hsp40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain . Members of the Hsp40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. In particular, Hdj1, the first human Hsp40/DnaJ protein identified, plays an important role in protein translation and folding, as well as in the regulation of Hsp70 function. HSP40 stimulates the ATPase activity of HSP70 which in turn causes conformational changes of the unfolded proteins. The Hsp40-Hsp70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1. Some studies have shown that the difference between HDJ1 and type 1 DNAJ proteins including HDJ2 and yeast YdjI is the result of the possession of a zinc finger domain by the latter, which helps in the function of protein folding.
Synonyms: DNAJ1, DNAJB1, HDJ1, Hsp40, HSPF1

Spezifität: ~40kDa. Species cross-reactivity: Yeast, does not cross react with human, mouse, rat.

Sensitivität: 0.5 µg/mL of SMC-166 was sufficient for detection of 50ng YDJ1 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Anwendungen

Applikationshinweise: 1:2000 (WB)

Konzentration: 1 mg/mL

Reinigung: Protein G Purified

Buffer: 50% glycerol

Lagerung: -20 °C

Storage Shipping Temp Max: Blue Ice or 4 °C

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Hsp40 (YDJ1- 2A7.H6), 0.1ug per lane