Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) Antikörper

Antigen: Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1)

Synonyme: ECGP, GP96, TRA1, GRP94, TA-3, Tra1, gp96, ERp99, Targ2, Tra-1, endoplasmin, Grp94, tra1, tra-1, fb61d09, wu:fb61d09, wu:fq25g01, Gp96, ppk98, ecgp, grp94, HSP90B1

Klonalität: Monoklonal (9G10)

Wirt: Ratte

Reaktivität: Human, Maus, Ratte (Rattus), Rind (Kuh), Hund, Huhn, Hamster, Pferd, Affe, Schwein, Kaninchen, Schaf, Xenopus laevis

Applikation: Western Blot (WB), Immunpräzipitation (IP), Durchflusszytometrie (FACS)

Produktnummer: ABIN361654

Produktbeschreibung

Produktmerkmale: Detects a 98kDa protein corresponding to the molecular mass of Grp94 on SDS PAGE immunoblots. Does not detect human Hsp90, Grp74, or GrpE from E.coli.

Weitere Bezeichnung: GRP94

Gen-ID: 7184

Swiss-Prot: P14625

Immunogen: Purified Grp94 isolated from chicken oviducts

Format: Liquid

Isotyp: IgG2a  (Passende Sekundärantikörper)

Klon: 9G10

Beschreibung: Grp94 (glucose regulated protein 94, gp96) is a constitutively expressed endoplasmic reticulum (ER) lumenal protein that is up-regulated in response to cellular stress such as heat shock, oxidative stress or glucose depletion. Grp94 is thought to play a role in protein translocation to the ER, in their subsequent folding and assembly, and in regulating protein secretion. Grp94 also plays a role in antigen presentation by accessing the endogenous pathway and eliciting specific CTL responses to chaperone bound peptides via MHC class I pathway Grp94 is a member of the Hsp90 family of stress proteins and shares sequence homolgy with its cytosolic equivalent, Hsp90. Both Hsp90 and Grp94 are calcium binding proteins. Despite sharing 50% sequence homology over its N domains and complete conservation in its ligand binding domains with Hsp90, Grp94 and Hsp90 differ in their interactions with regulatory ligands as Grp94 has weak ATP binding and hydrolyisis activity. Grp94 exists as a homodimer and the two subunits interact at two distinct intermolecular sites, C terminal dimerization domains and the N-terminal interacts with the middle domain of opposing subunits.. Grp94 contains a carboxy terminal KDEL (Lys-Asp-Glu-Leu) sequence which is believed to aid in its retention in the ER.
Synonyms: HSP90B1, GP96, TRA1, ECGP

Spezifität: Detects a 98kDa protein corresponding to the molecular mass of Grp94 on SDS PAGE immunoblots. Does not detect human Hsp90, Grp74 or GrpE from E. coli. Species cross-reactivity: Human, mouse, rat, bovine, canine, chicken, guinea pig, hamster, horse, monkey, pig, rabbit, sheep, Xenopus.

Sensitivität: 0.5 µg/mL of SMC-105 was sufficient for detection of Grp94 in 20µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.

Anwendungen

Konzentration: 1 mg/mL

Reinigung: Protein G Purified

Buffer: PBS pH7.2, 50% glycerol

Lagerung: -20 °C

Storage Shipping Temp Max: Blue Ice or 4 °C

Forschungsgebiet: Krebs, Organelle

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

GRP94 (9G10), heat shock Hela