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GRP94 Antikörper

HSP90B1 Reaktivität: Huhn WB, IF, FACS, IP, ICC Wirt: Ratte Monoclonal 9G10 unconjugated
Produktnummer ABIN361654
  • Target Alle GRP94 (HSP90B1) Antikörper anzeigen
    GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))
    Reaktivität
    • 133
    • 86
    • 55
    • 40
    • 35
    • 31
    • 30
    • 30
    • 28
    • 28
    • 26
    • 26
    • 25
    • 17
    • 10
    • 2
    • 2
    • 1
    • 1
    Huhn
    Wirt
    • 93
    • 40
    • 24
    • 2
    • 1
    Ratte
    Klonalität
    • 94
    • 66
    Monoklonal
    Konjugat
    • 70
    • 12
    • 11
    • 11
    • 10
    • 8
    • 4
    • 4
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser GRP94 Antikörper ist unkonjugiert
    Applikation
    • 152
    • 83
    • 69
    • 63
    • 49
    • 46
    • 39
    • 21
    • 13
    • 13
    • 12
    • 11
    • 4
    • 4
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC)
    Spezifität
    Detects ~98 kDa. Does not detect human HSP90, Grp74, or GrpE from E.coli.
    Kreuzreaktivität
    Huhn, Rind (Kuh), Hund, Meerschweinchen, Hamster, Pferd, Human, Affe, Maus, Schwein, Kaninchen, Ratte, Schaf, Xenopus laevis
    Aufreinigung
    Protein G Purified
    Immunogen
    Purified Grp94 isolated from chicken oviducts
    Klon
    9G10
    Isotyp
    IgG2a
    Top Product
    Discover our top product HSP90B1 Primärantikörper
  • Applikationshinweise
    • WB (1:2000)
    • ICC/IF (1:100)
    • optimal dilutions for assays should be determined by the user.
    Kommentare

    0.5 μg/ml of ABIN361653 was sufficient for detection of Grp94 in 20 μg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1 mg/mL
    Buffer
    PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    -20°C
  • Target
    GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))
    Andere Bezeichnung
    GRP94 (HSP90B1 Produkte)
    Synonyme
    ecgp antikoerper, gp96 antikoerper, tra1 antikoerper, grp94 antikoerper, DDBDRAFT_0206450 antikoerper, DDBDRAFT_0215015 antikoerper, DDB_0206450 antikoerper, DDB_0215015 antikoerper, TRA1 antikoerper, HSP90B1 antikoerper, ERp99 antikoerper, GRP94 antikoerper, TA-3 antikoerper, Targ2 antikoerper, Tra-1 antikoerper, Tra1 antikoerper, endoplasmin antikoerper, GP96 antikoerper, fb61d09 antikoerper, tra-1 antikoerper, wu:fb61d09 antikoerper, wu:fq25g01 antikoerper, Grp94 antikoerper, ECGP antikoerper, Gp96 antikoerper, ppk98 antikoerper, hsp108 antikoerper, heat shock protein 90kDa beta (Grp94), member 1 antikoerper, heat shock protein Hsp90 family protein antikoerper, 94 kD glucose-regulated protein antikoerper, heat shock protein 90 beta family member 1 antikoerper, heat shock protein 90, beta (Grp94), member 1 antikoerper, heat shock protein 90, beta (grp94), member 1 antikoerper, heat shock protein 90kDa beta family member 1 L homeolog antikoerper, hsp90b1 antikoerper, grp94 antikoerper, HSP90B1 antikoerper, Hsp90b1 antikoerper, hsp90b1.L antikoerper
    Hintergrund
    Grp94 (glucose regulated protein 94, gp96) is a constitutively expressed endoplasmic reticulum (ER) lumenal protein that is up-regulated in response to cellular stress such as heat shock, oxidative stress or glucose depletion. Grp94 is thought to play a role in protein translocation to the ER, in their subsequent folding and assembly, and in regulating protein secretion (1). Grp94 also plays a role in antigen presentation by accessing the endogenous pathway and eliciting specific CTL responses to chaperone bound peptides via MHC class I pathway (2). Grp94 is a member of the HSP90 family of stress proteins and shares sequence homology with its cytosolic equivalent, HSP90 (3). Both HSP90 and Grp94 are calcium binding proteins (4). Despite sharing 50 % sequence homology over its N domains and complete conservation in its ligand binding domains with HSP90, Grp94 and HSP90 differ in their interactions with regulatory ligands as Grp94 has weak ATP binding and hydrolysis activity (5). Grp94 exists as a homodimer and the two subunits interact at two distinct intermolecular sites, C terminal dimerization domains and the N-terminal interacts with the middle domain of opposing subunits (6). Grp94 contains a carboxy terminal KDEL (Lys-Asp-Glu-Leu) sequence which is believed to aid in its retention in the ER (7).
    Gen-ID
    7184
    NCBI Accession
    NP_003290
    UniProt
    P14625
    Pathways
    Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades
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