Heat Shock 27kDa Protein 1 (HSPB1) Antikörper

Antigen: Heat Shock 27kDa Protein 1 (HSPB1)

Synonyme: CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.76067, DKFZp586P1322, MKBP, Hs.78846, LOH11CR1K, MGC133245, 27kDa, Hsp27, hsp1, cb153, hsp25, hsp27, zgc:103437, MGC85307, HSPB1, cmt2f, hsp28, hs.76067, MGC147259

Klonalität: Monoklonal (5D12-A12)

Wirt: Maus

Reaktivität: Human

Applikation: Western Blot (WB), Immunpräzipitation (IP), ELISA, Immunhistochemie (IHC)

Produktnummer: ABIN361650

Produktbeschreibung

Produktmerkmale: Detects ~27kDa protiens corresponding to Hsp27 on SDS Page Immunoblots. Has no cross-reactivity to alphaB crystallin.

Weitere Bezeichnung: Hsp27

Gen-ID: 3315

Swiss-Prot: P04792

Immunogen: Human Hsp27

Kreuzreaktivität (Details): Very limited cross-reactivity to other species

Format: Liquid

Isotyp: IgG2b kappa  (Passende Sekundärantikörper)

Klon: 5D12-A12

Beschreibung: Hsp27s belong to an abundant and ubiquitous family of small heat shock proteins (sHSP). It is an important HSP found in both normal human cells and cancer cells. The basic structure of most sHsps is a homologous and highly conserved amino acid sequence, with an alpha-crystallin-domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers. Hsp27-oligomers consist of stable dimers formed by as many as 8-40 Hsp27 protein monomers. The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity. HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. Other functions include chaperone activity (as mentioned above), thermotolerance in vivo, inhibition of apoptosis, and signal transduction. Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. Hsp27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that hsp27 may serve some role in cross-bridge formation between actin and myosin. And finally, Hsp27 is also thought to be involved in the process of cell differentiation. The up-regulation of Hsp27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that Hsp27 may play a crucial role in termination of growth.
Synonyms: 28kDa heat shock protein, CMT2F, Hsp25, Hsp27, Hsp28, HspB1, SRP27

Spezifität: Detects a 27kDa protein corresponding to the molecular mass of hsp27 on SDS PAGE immunoblots. There is no cross-reactivity to alphaB-crystallin. Species cross-reactivity: Human. Very limited cross-reactivity to other species.

Sensitivität: 0.5 µg/mL of SMC-161 was sufficient for detection of Hsp27 in 10µg of HeLa lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Synonyme: CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.76067, DKFZp586P1322, MKBP, Hs.78846, LOH11CR1K, MGC133245, 27kDa, Hsp27, hsp1, cb153, hsp25, hsp27, zgc:103437, MGC85307, HSPB1, cmt2f, hsp28, hs.76067, MGC147259

Anwendungen

Applikationshinweise: 0.25-0.5 µg/mL (WB)

Konzentration: 1 mg/mL

Reinigung: Protein G Purified

Buffer: PBS pH7.4, 50% glycerol

Lagerung: -20 °C

Storage Shipping Temp Max: Blue Ice or 4 °C

Forschungsgebiet: Kardiovaskuläres System, Kontraktilität, Artherosklerose, Hitzeschockproteine, Zytoskelett, Mikrofilamente

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Hsp27(5D12-A3), cell lines