SOD2 Antikörper

Produktnummer ABIN361648

Produktdetails anti-SOD2 Antikörper

Target: SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SOD2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Spezifität: Detects ~25 kDa.

Kreuzreaktivität: Huhn, Rind (Kuh), Hund, Frosch, Wüstenrennmaus, Meerschweinchen, Hamster, Human, Affe, Maus, Schwein, Kaninchen, Ratte, Schaf, Xenopus laevis

Aufreinigung: Protein A Purified

Immunogen: Recombinant Human Mn SOD Protein

Anwendungsinformationen

Applikationshinweise:

• WB (1:5000)
• IHC (1:100)
• optimal dilutions for assays should be determined by the user.

Kommentare:

0.2 μg/ml of ABIN361647 was sufficient for detection of Mn SOD in 20 μg of rat brain tissue extract by colorimetric immunoblot analysis using Goat anti-mouse IgG:AP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Referenzen für anti-SOD2 Antikörper (ABIN361648)

Dawson, Katzenback, Storey: "Free-radical first responders: the characterization of CuZnSOD and MnSOD regulation during freezing of the freeze-tolerant North American wood frog, Rana sylvatica." in: Biochimica et biophysica acta, Vol. 1850, Issue 1, pp. 97-106, (2014) (PubMed).

Allan, Storey: "Expression of NF-?B and downstream antioxidant genes in skeletal muscle of hibernating ground squirrels, Spermophilus tridecemlineatus." in: Cell biochemistry and function, Vol. 30, Issue 2, pp. 166-74, (2012) (PubMed).

Ishii, Kunihiro, Tanaka, Hatano, Nishikata: "Cytosolic subunits of ATP synthase are localized to the cortical endoplasmic reticulum-rich domain of the ascidian egg myoplasm." in: Development, growth & differentiation, Vol. 54, Issue 8, pp. 753-66, (2012) (PubMed).

Details zu SOD2

Target: SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))

Andere Bezeichnung: SOD2 (SOD2 Produkte)

Synonyme: LOC100101896 antikoerper, MNSOD antikoerper, GB14346 antikoerper, sod2 antikoerper, MGC88869 antikoerper, Sod2 antikoerper, CG8905 antikoerper, Dmel\\CG8905 antikoerper, Mito SOD antikoerper, Mn SOD antikoerper, Mn-SOD antikoerper, Mn-SOD2 antikoerper, MnSOD antikoerper, MnSODII antikoerper, Mn[2+]SOD antikoerper, SOD antikoerper, SOD-2 antikoerper, SOD2 antikoerper, Sod-2 antikoerper, dSOD2 antikoerper, mitSOD2 antikoerper, mnSOD antikoerper, IPOB antikoerper, MVCD6 antikoerper, cb463 antikoerper, wu:fj33b01 antikoerper, zgc:73051 antikoerper, MnSOD antikoerper, manganese superoxide dismutase antikoerper, superoxide dismutase 2, mitochondrial antikoerper, superoxide dismutase 2 antikoerper, Mn superoxide dismutase antikoerper, Superoxide dismutase 2 (Mn) antikoerper, superoxide dismutase 2 L homeolog antikoerper, BDBG_07234 antikoerper, LOC100101896 antikoerper, MNSOD antikoerper, Sod2 antikoerper, sod2 antikoerper, LbMnSOD4 antikoerper, SOD2 antikoerper, LOC100282741 antikoerper, SOD-2 antikoerper, sod2.L antikoerper

Hintergrund: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (7). SOD3 can also be distinguished by its heparin-binding capacity (1).

Gen-ID: 6648

NCBI Accession: NP_000627

UniProt: P04179

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis, Negative Regulation of intrinsic apoptotic Signaling