Beta-Catenin (Phospho-Ser33,37) Antikörper

Antigen: Beta-Catenin (Phospho-Ser33,37)

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Western Blot (WB)

Produktnummer: ABIN361479

Produktbeschreibung

Format: Affinity-purified

Beschreibung: -catenin is a central component of the cadherin cell adhesion complex and plays an essential role in neural development in the Wingless/Wnt signaling pathway (Ding and Dale, 2002, Polakis, 2000). The role of -catenin in these processes is thought regulated by the sequential phosphorylation of Ser29 Ser33, Ser37 and Thr41 by glycogen synthase kinase 3 (GSK3 ) (Liu et al., 2002). This hyperphosphorylation promotes the ubiquitylation and targeted destruction of -catenin. Mutations in components of this phosphorylation regulated process that prevent -catenin hyperphosphorylation by GSK3 are strongly associated with cancers (Wang et al., 2003, Polakis, 2000, Liu et al., 2002). Anti-Phospho-Ser33,37 -Catenin Western blot of human embryonic kidney cell (HEK) lysate showing specific immunolabeling of the ~83k -catenin phosphorylated at Ser33 and Ser37 (Upper Panel) and a pan -catenin (Lower Panel). Control is a standard HEK cell lysate. GSK3 is a HEK cell lysate with glycogen synthase kinase 3 knocked down by SiRNA.

Spezifität: Specific for the ~83k ß-catenin phosphorylated at Ser33 and Ser37.

Anwendungen

Applikationshinweise: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Reinigung: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

Lagerung: Store at -20 °C, stable for 1 year

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blots of human embryonic kidney cell (HEK) lysate showing specific immunolabeling of the ~83k ß-catenin phosphorylated at Ser33 and Ser37 (Upper Panel) and a pan ß-catenin (Lower Panel). Control is a standard HEK cell lysate. GSK3 is a HEK cell lysate with glycogen synthase kinase 3ß knocked down by SiRNA.