Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
100 μL in 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.
Lagerung
-20 °C
Ahmed, Gardiner: "Preserving protein profiles in tissue samples: differing outcomes with and without heat stabilization." in: Journal of neuroscience methods, Vol. 196, Issue 1, pp. 99-106, (2011) (PubMed).
Target
CREB1
(cAMP Responsive Element Binding Protein 1 (CREB1))
creb antikoerper, CREB1 antikoerper, Creb1 antikoerper, CREB antikoerper, 2310001E10Rik antikoerper, 3526402H21Rik antikoerper, AV083133 antikoerper, Creb antikoerper, Creb-1 antikoerper, creb1 antikoerper, zgc:55598 antikoerper, cAMP responsive element binding protein 1 antikoerper, cAMP responsive element binding protein antikoerper, cAMP responsive element binding protein 1 S homeolog antikoerper, cAMP responsive element binding protein 1a antikoerper, CREB1 antikoerper, creb1 antikoerper, CREB antikoerper, Creb1 antikoerper, creb1.S antikoerper, creb1a antikoerper
Hintergrund
It is well known that the control of gene expression involves activation of protein kinase cascades that regulate transcription factors within the nucleus (Karin and Hunter, 1995). The cyclic AMP response element binding protein (CREB) is one of the best characterized stimulus-induced transcription factors (Montminy, 1997). This transcription factor is a component of intracellular signaling events that regulate a wide range of biological functions, from spermatogenesis to circadian rhythms and memory (Shaywitz and Greenberg, 1999, Silva et al., 1998). A variety of protein kinases including protein kinase A (PKA), mitogen-activated protein kinases (MAPKs), and Ca2+/calmodulin-dependent protein kinases (CaMKs) phosphorylate CREB at serine 133 (Ser133), and phosphorylation of Ser133 are required for CREB-mediated transcription (Johannessen et al., 2004, Kornhauser et al., 2002). Anti-Phospho-Ser133 CREB Western blot of a forskolin stimulated rat hippocampal lysate showing specific immunolabeling of the ~45k CREB phosphorylated at Ser133 (Left Lane). The right lane is indicative that the specific immunolabeling of the CREB protein is completely eliminated by treatment with lambda phosphatase ((-Ptase, 1200 units for 30 min).