Early Secretory Antigenic Target ESXA (ESAT-6) Antikörper

Antigen: Early Secretory Antigenic Target ESXA (ESAT-6)

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Bakterien

Applikation: Western Blot (WB)

Produktnummer: ABIN361308

Produktbeschreibung

Gen-ID: 886209

Immunogen: purified recombinant ESAT-6

Format: Affinity-purified

Isotyp: IgG

Anwendungen

Applikationshinweise: ESAT-6 antibody has been tested for use in Western Blotting. It is expected that the antibody will be suited for all applications where recognition of the native protein is required. A titration should be performed to determine the optimal concentration for use in each application.

Reinheit: > 95 % SDS

Reinigung: DEAE Sepharose, IMAC-Talon® resins.

Buffer: PBS + 0,1% NaN3

Lagerung: Store the antibody at -20°C. Avoid repeated freezing and thawing.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western Blotting analysis of recombinant protein ESAT-6 produced in Escherichia coli BL21 (lambdaDE3) transfected bacterial culture. SDS-PAGE Electrophoresis (Reducing conditions).Immunostaining with rabbit polyclonal antibody to ESAT6, dilution 0,5 µg/ml (3, 4), non purified serum (1, 2). Detection with goat anti-rabbit / HRP secondary antibody. Lane 1, 3: Escherichia coli non-transfected bacterial culture (cell lysate) - negative controls. Lane 2, 4: Escherichia coli transfected bacterial culture (cell lysate).