Ag85B Antikörper

Antigen: Ag85B

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Bakterien

Applikation: Western Blot (WB)

Produktnummer: ABIN361296

Produktbeschreibung

Gen-ID: 29027589

Immunogen: purified recombinant Ag85B

Format: Affinity-purified

Isotyp: IgG  (Passende Sekundärantikörper)

Anwendungen

Applikationshinweise: Ag85B antibody has been tested for use in Western Blotting. It is expected that the antibody will be suited for all applications where recognition of the native protein is required. A titration should be performed to determine the optimal concentration for use in each application.

Reinheit: > 95 % SDS

Buffer: PBS + 0,1% NaN3

Lagerung: Store the antibody at -20°C. Avoid repeated freezing and thawing.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western Blotting analysis of recombinant protein Ag85B produced in Escherichia coli BL21 (lambdaDE3) transfected bacterial culture. SDS-PAGE Electrophoresis (Reducing conditions).Immunostaining with rabbit polyclonal antibody to Ag85B, dilution 1,0 µg/m (3, 4), non purified serum (1, 2) l. Detection with goat anti-rabbit / HRP secondary antibody. Lane 2, 4: Escherichia coli transfected bacterial culture (cell lysate). Lane 1, 3: Escherichia coli non-transfected bacterial culture (cell lysate) - negative controls.