Actin alpha Antikörper

Antigen: Actin alpha

Synonyme: Actc-1

Klonalität: Monoklonal (33-32)

Wirt: Maus

Reaktivität: Human

Applikation: Western Blot (WB)

Produktnummer: ABIN361252

Produktbeschreibung

Produktmerkmale: Positive control: Mouse Heart.
Cellular Localization: Cytoplasma

Weitere Bezeichnung: alpha-Actin

Swiss-Prot: P68033/P68032

Immunogen: This antibody is produced by immunizing mouse with a synthetic peptide (KLH-coupled) corresponding to near N-terminal residues of alpha-Actin.

Kreuzreaktivität: Human, Maus, Ratte (Rattus)

Format: Liquid

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: 33-32

Beschreibung: Actins are highly conserved proteins expressed in all eucaryotic cells. Actin filaments form part of the cytoskeleton and play essential roles in regulating cell shape and movement. Six distinct actin isotypes have been identified in mammalian cells. Each is encoded by a separated gene and is expressed in a developmentally regulated and tissue-specific manner, alpha and beta cytoplasmic actins are expressed in a wide variety of cells, whereas, expression of alpha skeletal, alpha cardiac, alpha vascular, and gamma enteric actins are more restricted to specialized muscle cell type. Smooth muscle alpha actin is of further interest because it is one of a few genes whose expression is relatively restricted to vascular smooth muscle cells. Further more, expression of smooth muscle alpha actin is regulated by hormones, cell proliferation, and altered by pathological conditions including oncogenic transformation and atherosclerosis

Spezifität: Cellular Localization: Cytoplasma. Molecular Weight: 42kDa

Molekulargewicht: 42kDa

Anwendungen

Applikationshinweise: WB: 1:5,000

Konzentration: 0.5mg/ml

Buffer: 1xTBS (pH7.4), 0.5%BSA, 25%Glycerol.

Konservierungsmittel: 0.05% Sodium Azide.

Lagerung: Store at +4° C after thawing. Aliquot store at -20° C or -80° C. Avoid repeated freeze / thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis on mouse heart lysates using anti-α-Actin monoclonal antibody α-Actin,SMA