V5 Epitope Tag Antikörper

Details zu Produkt Nr. ABIN356537, Anbieter: Anmelden zum Anzeigen
Antigen
Reaktivität
Tag
399
18
16
3
3
Wirt
Kaninchen
248
135
34
7
5
4
3
1
1
Klonalität
Polyklonal
Konjugat
Unkonjugiert
30
27
21
13
12
12
11
11
11
10
10
10
10
9
8
8
7
6
5
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Applikation
Enzyme Immunoassay (EIA), Western Blotting (WB)
365
261
136
94
77
71
51
36
28
13
8
8
5
4
4
2
2
2
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen KLH conjugated synthetic peptide encoding the V5 tag (GKPIPNPLLGLDST) was used as antigen.
Sequenz GKPIPNPLLG LDST
Isotyp Ig
Reinigung Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Andere Bezeichnung Pk (V5) Epitope Tag (GKPIPNPLLGLDST)
Substanzklasse Tag
Hintergrund Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The V5 epitope tag is is composed of a 14 residue peptide, GKPIPNPLLGLDST, derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). This polyclonal antibody provides a simple solution to detect the expression of a V5-tagged protein in cells.
Forschungsgebiet Tags/Labels
Applikationshinweise ELISA: 1/1,000. Western Blot: 1/100-1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 0.25 mg/mL
Buffer PBS containing 0.09 % (w/v) Sodium Azide as preservative.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Bilder des Herstellers
 image for anti-V5 Epitope Tag antibody (ABIN356537) anti-V5 Epitope Tag antibody
 image for anti-V5 Epitope Tag antibody (ABIN356537) anti-V5 Epitope Tag antibody (Image 2)
Allgemeine Veröffentlichungen Dunn, ODowd, Randall: "Fine mapping of the binding sites of monoclonal antibodies raised against the Pk tag." in: Journal of immunological methods, Vol. 224, Issue 1-2, pp. 141-50, 1999 (PubMed).

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