Vacuolar Protein Sorting 25 Homolog (S. Cerevisiae) (VPS25) (internal) Antikörper

Antigen: Vacuolar Protein Sorting 25 Homolog (S. Cerevisiae) (VPS25)

Synonyme: DERP9, EAP20, FAP20, MGC10540, AW107467, D11Wsu68e, 1110020N13Rik, VPS25, dVps25, l(2)44Db, l(2)k08904, DmelCG14750, CG14750, vps25, MGC162560, im:6898134, MGC86224

Epitope: internal

Klonalität: Polyklonal

Wirt: Kaninchen

Applikation: Immunhistochemie (IHC), Western Blot (WB)

Produktnummer: ABIN351322

Produktbeschreibung

Weitere Bezeichnung: Vacuolar protein sorting-associated protein 25 (VPS25) (internal region)

Swiss-Prot: Q9CQ80

Immunogen: A synthetic peptide from the internal part of mouse Vacuolar protein sorting-associated protein 25 (VPS25) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat, zebra fish, xenopus and human.

Format: Lyophilized

Beschreibung: FUNCTION: Component of the ESCRT-II complex, which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery oftransmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note: Distributes diffusely throughout the cytoplasm and nucleoplasm, but exhibits a punctate distribution on coexpression with CHMP6. Also known as: VPS25.

Spezifität: Appears to be specific for VPS25.

Anwendungen

Applikationshinweise: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user.

Reinheit: whole serum

Lagerung: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Kamura, Burian, Khalili et al.: "Cloning and characterization of ELL-associated proteins EAP45 and EAP20. a role for yeast EAP-like proteins in regulation of gene expression by glucose." in: The Journal of biological chemistry, Vol. 276, Issue 19, pp. 16528-33, 2001 (PubMed).

von Schwedler, Stuchell, Mueller et al.: "The protein network of HIV budding." in: Cell, Vol. 114, Issue 6, pp. 701-13, 2003 (PubMed).

Gibbs, Weinstock, Metzker et al.: "Genome sequence of the Brown Norway rat yields insights into mammalian evolution." in: Nature, Vol. 428, Issue 6982, pp. 493-521, 2004 (PubMed).

Yorikawa, Shibata, Waguri et al.: "Human CHMP6, a myristoylated ESCRT-III protein, interacts directly with an ESCRT-II component EAP20 and regulates endosomal cargo sorting." in: The Biochemical journal, Vol. 387, Issue Pt 1, pp. 17-26, 2005 (PubMed).

Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).