Transient Receptor Potential Cation Channel, Subfamily V, Member 5 (TRPV5) (4th cytoplasmic loop) Antikörper

Antigen: Transient Receptor Potential Cation Channel, Subfamily V, Member 5 (TRPV5)

Synonyme: CAT2, ECAC1, OTRPC3, CaT2, Ecac1, MGC129346, MGC129347, D630033B11, TRPV5, ECAC

Epitope: 4th cytoplasmic loop

Klonalität: Polyklonal

Wirt: Schaf

Reaktivität: Kaninchen

Applikation: Immunhistochemie (IHC), Western Blot (WB)

Produktnummer: ABIN351298

Produktbeschreibung

Weitere Bezeichnung: TRPV5 (4th cytoplasmic loop)

Swiss-Prot: Q9XSM3

Immunogen: A synthetic peptide from the 4th cytoplasmic loop of rabbit TRPV5 conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Beschreibung: This gene is a member of the transient receptor family and the TrpV subfamily. The calcium-selective channel encoded by this gene has 6transmembrane-spanning domains, multiple potential phosphorylation sites, an N-linked glycosylation site, and 5 ANK repeats. This protein forms homotetramers or heterotetramers and is activated by a low internal calcium level. FUNCTION: Constitutively active calcium selective cation channel thought to be involved in Ca(2+) reabsorption in kidney and intestine. The channel is activated by low internal calcium level and the current exhibits an inward rectification. A Ca(2+)-dependent feedback regulation includes fast channel inactivation and slow current decay. Heteromeric assembly with TRPV6 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating. SUBCELLULAR LOCATION: Apical cell membrane; Multi-pass membrane protein. Note=Colocalized with S100A10 and ANAX2 along the apical domain of kidney distal tubular cells. TISSUE SPECIFICITY: Expressed at high levels in kidney, small intestine and pancreas, and at lower levels in testis, prostate, placenta, brain, colon and rectum. Also known as: TrpV5, osm-9-like TRP channel 3, OTRPC3, Epithelial calcium channel 1, ECaC, ECaC1, Calcium transport protein 2, CaT2, CAT2, ECAC1, OTRPC3, transient receptor potential cation channel subfamily V member 5.

Spezifität: Appears to be specific for TRPV5.

Anwendungen

Applikationshinweise: IHC, WB. A dilution of 1 : 300 to 1 : 4000 is recommended. The optimal dilution should be determined by the end user.

Reinheit: whole serum

Lagerung: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Hoenderop, van der Kemp, Hartog et al.: "Molecular identification of the apical Ca2+ channel in 1, 25-dihydroxyvitamin D3-responsive epithelia." in: The Journal of biological chemistry, Vol. 274, Issue 13, pp. 8375-8, 1999 (PubMed).

Nilius, Vennekens, Prenen et al.: "The single pore residue Asp542 determines Ca2+ permeation and Mg2+ block of the epithelial Ca2+ channel." in: The Journal of biological chemistry, Vol. 276, Issue 2, pp. 1020-5, 2001 (PubMed).

Hoenderop, Voets, Hoefs et al.: "Homo- and heterotetrameric architecture of the epithelial Ca2+ channels TRPV5 and TRPV6." in: The EMBO journal, Vol. 22, Issue 4, pp. 776-85, 2003 (PubMed).