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TrkA, B, C (pTyr683), (pTyr684) Antikörper

Reaktivität: Ratte WB, IHC Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN351071
  • Target
    TrkA, B, C
    Bindungsspezifität
    • 15
    • 15
    • 1
    • 1
    pTyr683, pTyr684
    Reaktivität
    • 39
    • 38
    • 24
    Ratte
    Wirt
    • 40
    Kaninchen
    Klonalität
    • 31
    • 9
    Polyklonal
    Konjugat
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    Unkonjugiert
    Applikation
    • 33
    • 27
    • 26
    • 7
    • 6
    • 6
    • 3
    Western Blotting (WB), Immunohistochemistry (IHC)
    Spezifität
    Specific for TrkA, TrkB, TrkC.
    Kreuzreaktivität
    Human, Maus, Ratte
    Kreuzreaktivität (Details)
    Other species not yet tested.
    Aufreinigung
    IgG
    Immunogen
    A synthetic peptide to phospho Y683 & Y684 of rat TrkA (and the corresponding phosphorylated sequences in TrkB and TrkC) conjugated to blue carrier protein was used as the immunogen. The antigen is homologous in mouse, human, chicken and zebrafish. Additionally, the antigen shares ~90% identity with the corresponding phosphorylated sequences in TrkB and TrkC.
    Isotyp
    IgG
  • Applikationshinweise
    IHC, WB. A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications. This antibody recognises also the corresponding phosphorylated sequences in TrkB,TrkC.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
    Handhabung
    Avoid freeze and thaw cycles.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
    Haltbarkeit
    12 months
  • Target
    TrkA, B, C
    Substanzklasse
    Tag
    Hintergrund
    FUNCTION: Required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). Known substrates for the Trk receptors are SHC1, PI 3-kinase, and PLC-gamma-1. Has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. Activates ERK1 by either SHC1- or PLC-gamma-1-dependent signaling pathway. CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. Binds SH2B2. Interacts with SQSTM1 which bridges NTRK1 to NGFR. Interacts with NGFR and ARMS. Can form a ternary complex with NGFR and ARMS and this complex is affected by the expression levels of ARMS. An increase in ARMS expression leads to a decreased association of NGFR and NTRK1. SUBCELLULAR LOCATION: Cell membrane, Single-pass type I membrane protein. Note: Endocytosed to the endosomes upon treatment of cells with NGF. TISSUE SPECIFICITY: Isoform Trka-II is primarily expressed in neuronal cells, isoform Trka-I is found in non-neuronal tissues.,Neurotrophic Receptors,High affinity nerve growth factor receptor, NTRK1, p140-TrkA, Trk-A, Neurotrophic tyrosine kinase receptor type 1, Slow nerve growth factor receptor
    UniProt
    P35739
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