Pan TrkA (Cytoplasmic Domain) Antikörper
Reaktivität: Maus
IHC, WB
Wirt: Kaninchen
Polyclonal
unconjugated
-
- Target
- Pan TrkA
- Bindungsspezifität
- Cytoplasmic Domain
- Reaktivität
- Maus
- Wirt
- Kaninchen
- Klonalität
- Polyklonal
- Applikation
- Immunohistochemistry (IHC), Western Blotting (WB)
- Spezifität
- Specific for TrkA.
- Kreuzreaktivität
- Human, Maus, Ratte
- Kreuzreaktivität (Details)
- Other species not yet tested.
- Aufreinigung
- IgG
- Immunogen
- A synthetic peptide from the cytoplasmic domain of mouse TrkA, TrkB, TrkC conjugated to blue carrier protein was used as the antigen. The antigen is homologous in rat, human and zebrafish.
- Isotyp
- IgG
-
- Applikationshinweise
- IHC, WB. A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications. This antibody recognises also TrkB,TrkC.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Lyophilized
- Rekonstitution
- Reconstitute in 500 μL of sterile water. Centrifuge to remove any insoluble material.
- Handhabung
- Avoid freeze and thaw cycles.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
- Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
- Haltbarkeit
- 12 months
-
- Target
- Pan TrkA
- Hintergrund
- FUNCTION: Required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). Known substrates for the Trk receptors are SHC1, PI 3-kinase, and PLC-gamma-1. Has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. Activates ERK1 by either SHC1- or PLC-gamma-1-dependent signaling pathway. CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. Binds SH2B2. Interacts with SQSTM1 which bridges NTRK1 to NGFR. Interacts with NGFR and ARMS. Can form a ternary complex with NGFR and ARMS and this complex is affected by the expression levels of ARMS. An increase in ARMS expression leads to a decreased association of NGFR and NTRK1. SUBCELLULAR LOCATION: Cell membrane, Single-pass type I membrane protein. Note: Endocytosed to the endosomes upon treatment of cells with NGF. TISSUE SPECIFICITY: Isoform Trka-II is primarily expressed in neuronal cells, isoform Trka-I is found in non-neuronal tissues.,Neurotrophic Receptors,High affinity nerve growth factor receptor, NTRK1, p140-TrkA, Trk-A, Neurotrophic tyrosine kinase receptor type 1, Slow nerve growth factor receptor
- UniProt
- Q3UFB7
-
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