Taste Receptor, Type 2, Member 43 (TAS2R43) Antikörper

Antigen: Taste Receptor, Type 2, Member 43 (TAS2R43)

Synonyme: T2R43, T2R52, T2R46, TAS2R43

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Immunhistochemie (IHC), Western Blot (WB)

Produktnummer: ABIN350981

Produktbeschreibung

Weitere Bezeichnung: taste receptor, type 2, member 43 (TAS2R43, T2R43, T2R52)

Swiss-Prot: P59537

Immunogen: A synthetic peptide from human taste receptor, type 2, member 43 (TAS2R43, T2R43, T2R52) conjugated to an immunogenic carrier protein was used as the immunogen. The antibody generated using this antigen is expected to also recognise TAS2R44, TAS2R45 and TAS2R47.

Format: Lyophilized

Beschreibung: FUNCTION: Receptor that may play a role in the perception of bitterness and is gustducin-linked. May play a role in sensing the chemical composition of the gastrointestinal content. The activity of this receptor may stimulate alpha gustducin, mediate PLC-beta-2 activation and lead to the gating of TRPM5. Activated by the sulfonyl amide sweeteners saccharin and acesulfame K. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Expressed in subsets of taste receptor cells of the tongue and exclusively in gustducin-positive cells. MISCELLANEOUS: Most taste cells may be activated by a limited number of bitter compounds; individual taste cells can discriminate among bitter stimuli.<||a href="http://senselab.med.yale.edu/senselab/ordb/" /> Also known as: Taste receptor type 2 member 43, T2R43, T2R52, TAS2R43.

Spezifität: Appears to be specific for TAS2R43 ( and also TAS2R44, TAS2R45 and TAS2R47. See the Immunogen)

Anwendungen

Applikationshinweise: IHC, WB (confirmed by recombinant protein). A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Reinheit: whole serum

Lagerung: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Chandrashekar, Mueller, Hoon et al.: "T2Rs function as bitter taste receptors." in: Cell, Vol. 100, Issue 6, pp. 703-11, 2000 (PubMed).

Margolskee: "Molecular mechanisms of bitter and sweet taste transduction." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 1-4, 2002 (PubMed).

Montmayeur, Matsunami: "Receptors for bitter and sweet taste." in: Current opinion in neurobiology, Vol. 12, Issue 4, pp. 366-71, 2002 (PubMed).

Bufe, Hofmann, Krautwurst et al.: "The human TAS2R16 receptor mediates bitter taste in response to beta-glucopyranosides." in: Nature genetics, Vol. 32, Issue 3, pp. 397-401, 2002 (PubMed).

Zhang, Hoon, Chandrashekar et al.: "Coding of sweet, bitter, and umami tastes: different receptor cells sharing similar signaling pathways." in: Cell, Vol. 112, Issue 3, pp. 293-301, 2003 (PubMed).

Conte, Ebeling, Marcuz et al.: "Identification and characterization of human taste receptor genes belonging to the TAS2R family." in: Cytogenetic and genome research, Vol. 98, Issue 1, pp. 45-53, 2003 (PubMed).

Kuhn, Bufe, Winnig et al.: "Bitter taste receptors for saccharin and acesulfame K." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 24, Issue 45, pp. 10260-5, 2004 (PubMed).