Synaptotagmin II (SYT2) (cytoplasmic domain Synaptotagmin II SytII SYT2) Antikörper

Antigen: Synaptotagmin II (SYT2)

Synonyme: SytII, FLJ42519, R74640, KIAA4194, mKIAA4194, SYNII, RATSYNII, si:ch211-236j4.1

Epitope: cytoplasmic domain Synaptotagmin II SytII SYT2

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Ratte (Rattus), Maus

Applikation: Immunhistochemie (IHC), Western Blot (WB)

Produktnummer: ABIN350927

Produktbeschreibung

Weitere Bezeichnung: Synaptotagmin-2 (Synaptotagmin II, SytII, SYT2) (cytoplasmic domain)

Swiss-Prot: P29101

Immunogen: A synthetic peptide from the cytoplasmic domain of rat, mouse Synaptotagmin-2 (Synaptotagmin II, SytII, SYT2) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Beschreibung: Synaptotagmins, like SYT2, are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. FUNCTION: May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. COFACTOR: Binds 3 calcium ions per subunit. The ions are bound to the C2 domains. SUBUNIT: Homotetramer. Interacts with stonin 2. SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle. Cytoplasmic vesicle, secretory vesicle, chromaffin granule. Note: Synaptic vesicles and chromaffin granules. Also known as: Synaptotagmin II, SytII, SYT2.

Spezifität: Appears to be specific for SYTII.

Anwendungen

Applikationshinweise: IHC, WB (confirmed by recombinant protein). A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Reinheit: whole serum

Lagerung: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

IF on 1 day- old mouse cortical cell culture using Rabbit antibody to rat, mouse Synaptotagmin-2 (Synaptotagmin II, SytII, SYT2): whole serum (ABIN350927) at 1: 2000 dilution. DAPI counter stained.

IHC on rat spinal cord using Rabbit antibody to rat, mouse Synaptotagmin-2 (Synaptotagmin II, SytII, SYT2): whole serum (ABIN350927) at 1 : 3000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).

IHC on rat spinal cord using Rabbit antibody to rat, mouse Synaptotagmin-2 (Synaptotagmin II, SytII, SYT2): whole serum (ABIN350927) at 1 : 3000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).

Publikationen

Publikationen: Geppert, Archer, Suedhof: "Synaptotagmin II. A novel differentially distributed form of synaptotagmin." in: The Journal of biological chemistry, Vol. 266, Issue 21, pp. 13548-52, 1991 (PubMed).

Fukuda, Aruga, Niinobe et al.: "Inositol-1,3,4,5-tetrakisphosphate binding to C2B domain of IP4BP/synaptotagmin II." in: The Journal of biological chemistry, Vol. 269, Issue 46, pp. 29206-11, 1994 (PubMed).

Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).