Synapstotagmin I (SYT1) (N-Term Synaptotagmin I SytI p65 SVP65) Antikörper

Antigen: Synapstotagmin I (SYT1)

Synonyme: P65, SYT, SVP65, DKFZp781D2042, AW124717, G630098F17Rik, syt, svp65, DKFZP459P193, SYT1, SytI, p65

Epitope: N-Term Synaptotagmin I SytI p65 SVP65

Klonalität: Polyklonal

Wirt: Kaninchen

Applikation: Immunhistochemie (IHC), Western Blot (WB)

Produktnummer: ABIN350919

Produktbeschreibung

Weitere Bezeichnung: Synaptotagmin-1 (Synaptotagmin I, SytI, p65, SVP65) (n-terminal region)

Swiss-Prot: P21707

Immunogen: A synthetic peptide from rat Synaptotagmin-1 (Synaptotagmin I, SytI, p65, SVP65) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in mouse.

Format: Lyophilized

Beschreibung: FUNCTION: May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2. COFACTOR: Binds 3 calcium ions per subunit. The ions are bound to the C2 domains. SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle. Cytoplasmic vesicle, secretory vesicle, chromaffin granule. Note: Synaptic vesicles and chromaffin granules. TISSUE SPECIFICITY: Predominantly expressed in rostral, phylogenetically younger brain regions, and in some endocrine tissues. Also known as: Synaptotagmin-1, Synaptotagmin I, SytI, p65, SVP65.

Spezifität: Appears to be specific for SYTI.

Anwendungen

Applikationshinweise: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Reinheit: whole serum

Lagerung: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

WB on rat brain lysate using Rabbit antibody to rat, mouse Synaptotagmin-1 (Synaptotagmin I, SytI, p65, SVP65): whole serum (ABIN350919) at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the detected band.

IF on 1 day- old mouse cortical cell culture using Rabbit antibody to rat, mouse Synaptotagmin-1 (Synaptotagmin I, SytI, p65, SVP65): whole serum (ABIN350919) at 1: 2000 dilution. DAPI counter stained.

IF on 1 day- old mouse cortical cell culture using Rabbit antibody to rat, mouse Synaptotagmin-1 (Synaptotagmin I, SytI, p65, SVP65): whole serum (ABIN350919) at 1: 2000 dilution. DAPI counter stained.

Publikationen

Publikationen: Sutton, Davletov, Berghuis et al.: "Structure of the first C2 domain of synaptotagmin I: a novel Ca2+/phospholipid-binding fold." in: Cell, Vol. 80, Issue 6, pp. 929-38, 1995 (PubMed).

Schivell, Batchelor, Bajjalieh: "Isoform-specific, calcium-regulated interaction of the synaptic vesicle proteins SV2 and synaptotagmin." in: The Journal of biological chemistry, Vol. 271, Issue 44, pp. 27770-5, 1996 (PubMed).

Shao, Fernandez, Suedhof et al.: "Solution structures of the Ca2+-free and Ca2+-bound C2A domain of synaptotagmin I: does Ca2+ induce a conformational change?" in: Biochemistry, Vol. 37, Issue 46, pp. 16106-15, 1998 (PubMed).

Coppola, Magnin-Luthi, Perret-Menoud et al.: "Direct interaction of the Rab3 effector RIM with Ca2+ channels, SNAP-25, and synaptotagmin." in: The Journal of biological chemistry, Vol. 276, Issue 35, pp. 32756-62, 2001 (PubMed).

Fernandez, Arauc, Ubach et al.: "Three-dimensional structure of the synaptotagmin 1 C2B-domain: synaptotagmin 1 as a phospholipid binding machine." in: Neuron, Vol. 32, Issue 6, pp. 1057-69, 2001 (PubMed).

Heindel, Schmidt, Veit: "Palmitoylation sites and processing of synaptotagmin I, the putative calcium sensor for neurosecretion." in: FEBS letters, Vol. 544, Issue 1-3, pp. 57-62, 2003 (PubMed).

Schivell, Mochida, Kensel-Hammes et al.: "SV2A and SV2C contain a unique synaptotagmin-binding site." in: Molecular and cellular neurosciences, Vol. 29, Issue 1, pp. 56-64, 2005 (PubMed).