Synaptosomal-Associated Protein, 25kDa (SNAP25) Antikörper

Antigen: Synaptosomal-Associated Protein, 25kDa (SNAP25)

Synonyme: RIC4, SEC9, SNAP, RIC-4, SNAP-25, FLJ23079, bA416N4.2, dJ1068F16.2, ric4, sec9, ric-4, snap-25, MGC68863, SNAP25, MGC139754, DKFZp459B0611, DKFZp459G0410, sp, Bdr, SNAP-25B, MGC105414, snap25

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Ratte (Rattus), Maus

Applikation: Immunhistochemie (IHC), Western Blot (WB)

Produktnummer: ABIN350905

Produktbeschreibung

Weitere Bezeichnung: Synaptosomal-associated protein 25 (SNAP-25,SNAP25, SEC9, RIC-4)

Swiss-Prot: P60881

Immunogen: A synthetic peptide from rat Synaptosomal-associated protein 25 (SNAP-25, SNAP25, SEC9, RIC-4) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in mouse.

Format: Lyophilized

Beschreibung: FUNCTION: t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. SUBUNIT: Part of the SNARE core complex containingSNAP25, VAMP2 and STX1A. This complex binds CPLX1. Interacts with TRIM9, RIMS1,SNAP25BP and HGS. Binds STXBP6. Found in a ternary complex with STX1A and VAMP8. Found in a complex containing SYT1, SV2B and syntaxin-1. SUBCELLULAR LOCATION: Membrane; Lipid-anchor. Cell junction, synapse, synaptosome. Note: Membrane association requires palmitoylation. TISSUE SPECIFICITY: Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum. Also known as:SNAP-25, Synaptosomal-associated 25 kDa protein,SNAP25, RIC4, SEC9,SNAP, RIC-4, Super protein, SUP.

Spezifität: Appears to be specific for SNAP-25.

Anwendungen

Applikationshinweise: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Reinheit: whole serum

Lagerung: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

WB on rat brain lysate using Rabbit antibody to rat, mouse Synaptosomal-associated protein 25 (SNAP-25, SNAP25, SEC9, RIC-4): whole serum (ABIN350905) at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the detected band.

Publikationen

Publikationen: Hess, Slater, Wilson et al.: "The 25 kDa synaptosomal-associated protein SNAP-25 is the major methionine-rich polypeptide in rapid axonal transport and a major substrate for palmitoylation in adult CNS." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 12, Issue 12, pp. 4634-41, 1993 (PubMed).

Jacobsson, Piehl, Bark et al.: "Differential subcellular localization of SNAP-25a and SNAP-25b RNA transcripts in spinal motoneurons and plasticity in expression after nerve injury." in: Brain research. Molecular brain research, Vol. 37, Issue 1-2, pp. 49-62, 1996 (PubMed).

Bean, Seifert, Chen et al.: "Hrs-2 is an ATPase implicated in calcium-regulated secretion." in: Nature, Vol. 385, Issue 6619, pp. 826-9, 1997 (PubMed).

Sutton, Fasshauer, Jahn et al.: "Crystal structure of a SNARE complex involved in synaptic exocytosis at 2.4 A resolution." in: Nature, Vol. 395, Issue 6700, pp. 347-53, 1998 (PubMed).

Fasshauer, Antonin, Margittai et al.: "Mixed and non-cognate SNARE complexes. Characterization of assembly and biophysical properties." in: The Journal of biological chemistry, Vol. 274, Issue 22, pp. 15440-6, 1999 (PubMed).

Bouverat, Krueger, Coetzee et al.: "Expression of rab GTP-binding proteins during oligodendrocyte differentiation in culture." in: Journal of neuroscience research, Vol. 59, Issue 3, pp. 446-53, 2000 (PubMed).

Coppola, Magnin-Luthi, Perret-Menoud et al.: "Direct interaction of the Rab3 effector RIM with Ca2+ channels, SNAP-25, and synaptotagmin." in: The Journal of biological chemistry, Vol. 276, Issue 35, pp. 32756-62, 2001 (PubMed).

Scales, Hesser, Masuda et al.: "Amisyn, a novel syntaxin-binding protein that may regulate SNARE complex assembly." in: The Journal of biological chemistry, Vol. 277, Issue 31, pp. 28271-9, 2002 (PubMed).

Hepp, Cabaniols, Roche: "Differential phosphorylation of SNAP-25 in vivo by protein kinase C and protein kinase A." in: FEBS letters, Vol. 532, Issue 1-2, pp. 52-6, 2002 (PubMed).

Ernst, Brunger: "High resolution structure, stability, and synaptotagmin binding of a truncated neuronal SNARE complex." in: The Journal of biological chemistry, Vol. 278, Issue 10, pp. 8630-6, 2003 (PubMed).